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MXI1 Recombinant antibody, PBS Only (Detector)

MXI1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242089D2

Cat no : 84733-5-PBS

Synonyms

MAX interactor 1, Max interacting protein 1, MAD2, Class C basic helix-loop-helix protein 11, bHLHc11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MXI1 Recombinant antibody (84733-5-RR)

    WB analysis using 84733-5-RR (same clone as 84733-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84733-5-RR (MXI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84733-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01509-3

    Sandwich ELISA standard curve of MP01509-3

    Sandwich ELISA standard curve of MP01509-3, Human MXI1 Recombinant Matched Antibody Pair - PBS only. 84733-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3024. 84733-5-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL

  • Sample test of MP01509-3

    Sample test of MP01509-3

    The mean MXI1 concentration was determined to be 20.19 ng/mL in HeLa cell extract based on a 1.0 mg/mL extract load.

  • Flow cytometry (FC) experiment of HeLa cells using MXI1 Recombinant antibody (84733-5-RR)

    FC experiment of HeLa using 84733-5-RR (same clone as 84733-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MXI1 Recombinant antibody (84733-5-RR, Clone:242089D2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84733-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84733-5-RR

    Affinity and Kinetic Characterization of 84733-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84733-5-RR against Human MXI1 were performed. The affinity constant is below 1 pM.

Product Information

84733-5-PBS targets MXI1 as part of a matched antibody pair:

MP01509-3: 84733-1-PBS capture and 84733-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MXI1 fusion protein Ag3024
Full Name MAX interactor 1
Calculated Molecular Weight228 aa, 26 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC012907
Gene Symbol MXI1
Gene ID (NCBI) 4601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MXI1 is a transcription factor containing a basic helix-loop-helix leucine zipper (bHLHzip) and belongs to the Myc-Max-Mad transcriptional network. It is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors.