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SAE1 Recombinant antibody

SAE1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241923F12

Cat no : 84516-6-RR

Synonyms

SUMO-activating enzyme subunit 1, SUA1, Sentrin/SUMO activating protein AOS1, HSPC140, AOS1

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SAE1 Recombinant antibody (84516-6-RR)

    IF Staining of HeLa using 84516-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SAE1 antibody (84516-6-RR, Clone: 241923F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SAE1 Recombinant antibody (84516-6-RR)

    IF Staining of MCF-7 using 84516-6-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SAE1 antibody (84516-6-RR, Clone: 241923F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive IF/ICC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84516-6-RR is SAE1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SAE1 fusion protein Ag0275
Full Name SUMO1 activating enzyme subunit 1
Calculated Molecular Weight 38 kDa
GenBank Accession NumberBC000344
Gene Symbol SAE1
Gene ID (NCBI) 10055
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).

Protocols

Product Specific Protocols
IF protocol for SAE1 antibody 84516-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols