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Cyclin E Recombinant antibody, PBS Only (Detector)

Cyclin E Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240136G9

Cat no : 82700-8-PBS

Synonyms

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E1, CCNE

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00361-2

    Sandwich ELISA standard curve of MP00361-2

    Sandwich ELISA standard curve of MP00361-2, Human Cyclin Monoclonal Matched Antibody Pair - PBS only. 82700-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2110. 82700-8-PBS was HRP conjugated as the detection antibody. Range: 0.313-40 ng/mL.

  • Sample test of MP00361-2

    Sample test of MP00361-2

    The mean human Cyclin E concentration was determined to be 132.4 ng/mL in Hela cell extract based on a 3.0 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cyclin E Recombinant antibody (82700-8-RR)

    IF Staining of HepG2 using 82700-8-RR (same clone as 82700-8-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E antibody (82700-8-RR, Clone: 240136G9 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using Cyclin E Recombinant antibody (82700-8-RR)

    FC experiment of MCF-7 using 82700-8-RR (same clone as 82700-8-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug Cyclin E Recombinant antibody (82700-8-RR, Clone:240136G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Cyclin E Recombinant antibody (82700-8-RR)

    FC experiment of HeLa using 82700-8-RR (same clone as 82700-8-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant antibody (82700-8-RR, Clone:240136G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00361-2

    Cytometric bead array standard curve of MP00361-2

    Cytometric bead array standard curve of MP00361-2, Cyclin E Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82700-6-PBS. Detection antibody: 82700-8-PBS. Standard: Ag2110. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00361-3

    Cytometric bead array standard curve of MP00361-3

    Cytometric bead array standard curve of MP00361-3, Cyclin E Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82700-5-PBS. Detection antibody: 82700-8-PBS. Standard: Ag2110. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 82700-8-RR

    Affinity and Kinetic Characterization of 82700-8-RR

    Biolayer interferometry (BLl) kinetic assays of 82700-8-RR against Human Cyclin E were performed. The affinity constant is 0.229 nM.

Product Information

82700-8-PBS targets Cyclin E as part of a matched antibody pair:

MP00361-2: 82700-6-PBS capture and 82700-8-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00361-3: 82700-5-PBS capture and 82700-8-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cyclin E fusion protein Ag2110
Full Name cyclin E1
Calculated Molecular Weight 410 aa, 47 kDa
GenBank Accession NumberBC035498
Gene Symbol Cyclin E1
Gene ID (NCBI) 898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.