Cyclin E Recombinant antibody

Cyclin E Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240136G9

Cat no : 82700-8-RR

Synonyms

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E1, CCNE

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Validation Data Gallery

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  IF Staining of HepG2 using 82700-8-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E antibody (82700-8-RR, Clone: 240136G9 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of MCF-7 using 82700-8-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug Cyclin E Recombinant antibody (82700-8-RR, Clone:240136G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of HeLa using 82700-8-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant antibody (82700-8-RR, Clone:240136G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 82700-8-RR

  Biolayer interferometry (BLl) kinetic assays of 82700-8-RR against Human Cyclin E were performed. The affinity constant is 0.229 nM.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82700-8-RR is Cyclin E Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cyclin E fusion protein Ag2110
• Full Name: cyclin E1
• Calculated Molecular Weight: 410 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC035498
• Gene Symbol: Cyclin E1
• Gene ID (NCBI): 898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).