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RTN3 Monoclonal antibody, PBS Only (Detector)

RTN3 Monoclonal Antibody for WB, IP, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IP, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E3H11

Cat no : 68215-1-PBS

Synonyms

ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RTN3 Monoclonal antibody (68215-1-Ig)

    WB analysis using 68215-1-Ig (same clone as 68215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68215-1-Ig (RTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68215-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50025-1

    Sandwich ELISA standard curve of MP50025-1

    Sandwich ELISA standard curve ofMP50025-1, RTN3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68215-2-PBS. Detection antibody: HRP-conjugated 68215-1-PBS. Standard: Ag13766. Range: 0.625-20 ng/mL.

  • Immunoprecipitation (IP) experiment of A549 cells using RTN3 Monoclonal antibody (68215-1-Ig)

    IP experiment of A549 using 68215-1-Ig (same clone as 68215-1-PBS)

    IP result of anti-RTN3 (IP:68215-1-Ig, 4ug; Detection:68215-1-Ig 1:2000) with A549 cells lysate 840 ug. This data was developed using the same antibody clone with 68215-1-PBS in a different storage buffer formulation.

Product Information

68215-1-PBS targets RTN3 as part of a matched antibody pair:

MP50025-1: 68215-2-PBS capture and 68215-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RTN3 fusion protein Ag13766
Full Name reticulon 3
Calculated Molecular Weight 113 kDa
Observed Molecular Weight25-28 kDa
GenBank Accession NumberBC011394
Gene Symbol RTN3
Gene ID (NCBI) 10313
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.