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RTN3 Monoclonal antibody

RTN3 Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1E3H11

Cat no : 68215-1-Ig

Synonyms

ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RTN3 Monoclonal antibody (68215-1-Ig)

    WB analysis using 68215-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68215-1-Ig (RTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunoprecipitation (IP) experiment of A549 cells using RTN3 Monoclonal antibody (68215-1-Ig)

    IP experiment of A549 using 68215-1-Ig

    IP result of anti-RTN3 (IP:68215-1-Ig, 4ug; Detection:68215-1-Ig 1:2000) with A549 cells lysate 840 ug.

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IP detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68215-1-Ig targets RTN3 in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RTN3 fusion protein Ag13766
Full Name reticulon 3
Calculated Molecular Weight 113 kDa
Observed Molecular Weight25-28 kDa
GenBank Accession NumberBC011394
Gene Symbol RTN3
Gene ID (NCBI) 10313
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.

Protocols

Product Specific Protocols
WB protocol for RTN3 antibody 68215-1-IgDownload protocol
IP protocol for RTN3 antibody 68215-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols