RTN3 Monoclonal antibody

RTN3 Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1E3H11

Cat no : 68215-1-Ig

Synonyms

ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1

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Validation Data Gallery

• 

  WB analysis using 68215-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68215-1-Ig (RTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  IP experiment of A549 using 68215-1-Ig

  IP result of anti-RTN3 (IP:68215-1-Ig, 4ug; Detection:68215-1-Ig 1:2000) with A549 cells lysate 840 ug.

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IP detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68215-1-Ig targets RTN3 in WB, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RTN3 fusion protein Ag13766
• Full Name: reticulon 3
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC011394
• Gene Symbol: RTN3
• Gene ID (NCBI): 10313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.