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HSPB3 Monoclonal antibody

HSPB3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2A10B5

Cat no : 67890-1-Ig

Synonyms

HSPL27, HSP27, HSP 17, Heat shock protein family B member 3, Heat shock protein beta-3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPB3 Monoclonal antibody (67890-1-Ig)

    WB analysis using 67890-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of human heart tissue using HSPB3 Monoclonal antibody (67890-1-Ig)

    WB analysis of human heart using 67890-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using HSPB3 Monoclonal antibody (67890-1-Ig)

    WB analysis of mouse heart using 67890-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit heart tissue using HSPB3 Monoclonal antibody (67890-1-Ig)

    WB analysis of rabbit heart using 67890-1-Ig

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HSPB3 Monoclonal antibody (67890-1-Ig)

    IHC staining of mouse kidney using 67890-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HSPB3 Monoclonal antibody (67890-1-Ig)

    IHC staining of mouse kidney using 67890-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HSPB3 Monoclonal antibody (67890-1-Ig)

    IHC staining of mouse kidney using 67890-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using HSPB3 Monoclonal antibody (67890-1-Ig)

    IHC staining of rat kidney using 67890-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using HSPB3 Monoclonal antibody (67890-1-Ig)

    IHC staining of rat kidney using 67890-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using HSPB3 Monoclonal antibody (67890-1-Ig)

    IHC staining of rat kidney using 67890-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSPB3 Monoclonal antibody (67890-1-Ig)

    IF Staining of HeLa using 67890-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPB3 antibody (67890-1-Ig, Clone: 2A10B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSPB3 Monoclonal antibody (67890-1-Ig)

    IF Staining of HeLa using 67890-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPB3 antibody (67890-1-Ig, Clone: 2A10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using HSPB3 Monoclonal antibody (67890-1-Ig)

    FC experiment of HeLa using 67890-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human HSPB3 (67890-1-Ig, Clone:2A10B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhuman heart tissue, mouse heart tissue, rabbit heart tissue, rat heart tissue
Positive IHC detected inrat kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67890-1-Ig targets HSPB3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HSPB3 fusion protein Ag26940
Full Name heat shock 27kDa protein 3
Calculated Molecular Weight 150 aa, 17 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC126272
Gene Symbol HSPB3
Gene ID (NCBI) 8988
RRIDAB_2918646
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heat shock protein family B (small) member 3 (HSPB3) is the third member of the sHSP family in human and is mainly expressed in skeletal and smooth muscles, and can associate with HSPB2, which is another sHSP prominently expressed in cardiac and skeletal muscles. HSPB3 is involved in several cellular functions such as cell signaling, differentiation, and apoptosis, and mutations of HSPB3 are associated with neuromuscular diseases, such as axonal Charcot‐Marie‐Tooth disease (PMID: 31709619) (PMID: 32093037). The molecular weight of HSPB3 is about 17 kDa.

Protocols

Product Specific Protocols
WB protocol for HSPB3 antibody 67890-1-IgDownload protocol
IHC protocol for HSPB3 antibody 67890-1-IgDownload protocol
IF protocol for HSPB3 antibody 67890-1-IgDownload protocol
FC protocol for HSPB3 antibody 67890-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols