HSPB3 Monoclonal antibody, PBS Only

HSPB3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

rabbit, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A10B5

Cat no : 67890-1-PBS

Synonyms

HSPL27, HSP27, HSP 17, Heat shock protein family B member 3, Heat shock protein beta-3

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Validation Data Gallery

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  WB analysis using 67890-1-Ig (same clone as 67890-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 67890-1-Ig (same clone as 67890-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 67890-1-Ig (same clone as 67890-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit heart using 67890-1-Ig (same clone as 67890-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67890-1-Ig (same clone as 67890-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67890-1-Ig (same clone as 67890-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67890-1-Ig (same clone as 67890-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 67890-1-Ig (same clone as 67890-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 67890-1-Ig (same clone as 67890-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 67890-1-Ig (same clone as 67890-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67890-1-Ig (same clone as 67890-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPB3 antibody (67890-1-Ig, Clone: 2A10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67890-1-Ig (same clone as 67890-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPB3 antibody (67890-1-Ig, Clone: 2A10B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67890-1-Ig (same clone as 67890-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human HSPB3 (67890-1-Ig, Clone:2A10B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67890-1-PBS in a different storage buffer formulation.

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Product Information

67890-1-PBS targets HSPB3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rabbit, rat, mouse, human samples.

• Tested Reactivity: rabbit, rat, mouse, human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSPB3 fusion protein Ag26940
• Full Name: heat shock 27kDa protein 3
• Calculated Molecular Weight: 150 aa, 17 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC126272
• Gene Symbol: HSPB3
• Gene ID (NCBI): 8988
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Heat shock protein family B (small) member 3 (HSPB3) is the third member of the sHSP family in human and is mainly expressed in skeletal and smooth muscles, and can associate with HSPB2, which is another sHSP prominently expressed in cardiac and skeletal muscles. HSPB3 is involved in several cellular functions such as cell signaling, differentiation, and apoptosis, and mutations of HSPB3 are associated with neuromuscular diseases, such as axonal Charcot‐Marie‐Tooth disease (PMID: 31709619) (PMID: 32093037). The molecular weight of HSPB3 is about 17 kDa.