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CYP2W1 Monoclonal antibody

CYP2W1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2B10H2

Cat no : 67419-1-Ig

Synonyms

EC:1.14.14.-, Cytochrome P450 2W1, CYPIIW1, 2B10H2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CYP2W1 Monoclonal antibody (67419-1-Ig)

    WB analysis of HEK-293 using 67419-1-Ig

    WB result of CYP2W1 antibody (67419-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP2W1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using CYP2W1 Monoclonal antibody (67419-1-Ig)

    WB analysis using 67419-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67419-1-Ig (CYP2W1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CYP2W1 Monoclonal antibody (67419-1-Ig)

    IHC staining of human colon cancer using 67419-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67419-1-Ig (CYP2W1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CYP2W1 Monoclonal antibody (67419-1-Ig)

    IF Staining of HepG2 using 67419-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP2W1 antibody (67419-1-Ig, Clone: 2B10H2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HUVEC cells using CYP2W1 Monoclonal antibody (67419-1-Ig)

    FC experiment of HUVEC using 67419-1-Ig

    1x10^6 HUVEC cells were intracellularly stained with 0.4 ug CYP2W1 Monoclonal antibody (67419-1-Ig, Clone:2B10H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67419-1-Ig targets CYP2W1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP2W1 fusion protein Ag29982
Full Name cytochrome P450, family 2, subfamily W, polypeptide 1
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 45-54 kDa
GenBank Accession NumberBC025761
Gene Symbol CYP2W1
Gene ID (NCBI) 54905
RRIDAB_2882659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome P450s have been proposed to be of importance both in carcinogenesis, by activating precarcinogens, and as determinants of cancer chemotherapy, where they participate in activation or inactivation of anti-cancer drugs. CYP2W1 is exclusively expressed in transformed tissue in the adult human, mainly in colon tumors. It is a potential drug target in cancer therapy. CYP2W1 has a molecular weight of 52-54 kDa, and 30/45 kDa of potential isoforms.

Protocols

Product Specific Protocols
WB protocol for CYP2W1 antibody 67419-1-IgDownload protocol
IHC protocol for CYP2W1 antibody 67419-1-IgDownload protocol
IF protocol for CYP2W1 antibody 67419-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols