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IMP3 Monoclonal antibody

IMP3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E7D6

Cat no : 66247-1-Ig

Synonyms

MRPS4, IMP 3, C15orf12, BRMS2, 1E7D6

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IMP3 Monoclonal antibody (66247-1-Ig)

    WB analysis of HeLa using 66247-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66247-1-Ig (IMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using IMP3 Monoclonal antibody (66247-1-Ig)

    WB analysis of HepG2 using 66247-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66247-1-Ig (IMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using IMP3 Monoclonal antibody (66247-1-Ig)

    WB analysis of SKOV-3 using 66247-1-Ig

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66247-1-Ig (IMP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IMP3 Monoclonal antibody (66247-1-Ig)

    IHC staining of human breast cancer using 66247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IMP3 Monoclonal antibody (66247-1-Ig)

    IHC staining of human breast cancer using 66247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IMP3 Monoclonal antibody (66247-1-Ig)

    IHC staining of human breast cancer using 66247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IMP3 Monoclonal antibody (66247-1-Ig)

    IHC staining of human breast cancer using 66247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IMP3 Monoclonal antibody (66247-1-Ig)

    IF Staining of HepG2 using 66247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IMP3 Monoclonal antibody (66247-1-Ig)

    IF Staining of HepG2 using 66247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IMP3 Monoclonal antibody (66247-1-Ig)

    IF Staining of HepG2 using 66247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using IMP3 Monoclonal antibody (66247-1-Ig)

    FC experiment of HepG2 using 66247-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human IMP3 (66247-1-Ig, Clone:1E7D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, SKOV-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66247-1-Ig targets IMP3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IMP3 fusion protein Ag23602
Full Name IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
Calculated Molecular Weight 184 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC020557
Gene Symbol IMP3
Gene ID (NCBI) 55272
RRIDAB_2881636
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMP3 is a member of the insulin-like growth factor (IGF-II) mRNA binding protein (IMP) family that consist of IMP1, IMP2, and IMP3. IMP3 is an oncofetal protein associated with aggressive and advanced tumors and is specifically expressed in malignant tumors but not found in benign tissues [PMID:22497850]. IMP3 forms a ternary complex with IMP4 and MPP10 (MPHOSPH10) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing[PMID: 12655004].

Protocols

Product Specific Protocols
WB protocol for IMP3 antibody 66247-1-IgDownload protocol
IHC protocol for IMP3 antibody 66247-1-IgDownload protocol
IF protocol for IMP3 antibody 66247-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols