Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of HeLa using 66247-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 66247-1-Ig (IMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 66247-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 66247-1-Ig (IMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 66247-1-Ig
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66247-1-Ig (IMP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 66247-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66247-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66247-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66247-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 66247-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 66247-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 66247-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
FC experiment of HepG2 using 66247-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human IMP3 (66247-1-Ig, Clone:1E7D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

IMP3 Monoclonal antibody

Name: IMP3 Monoclonal antibody
Brand: Proteintech
SKU: 66247-1-Ig

IMP3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 66247-1-Ig

Clone No.1E7D6

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

MRPS4, IMP 3, C15orf12, BRMS2, 1E7D6

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, SKOV-3 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66247-1-Ig targets IMP3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	IMP3 fusion protein Ag23602 Predict reactive species
Full Name	IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
Calculated Molecular Weight	184 aa, 22 kDa
Observed Molecular Weight	22 kDa
GenBank Accession Number	BC020557
Gene Symbol	IMP3
Gene ID (NCBI)	55272
RRID	AB_2881636
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9NV31
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMP3 is a member of the insulin-like growth factor (IGF-II) mRNA binding protein (IMP) family that consist of IMP1, IMP2, and IMP3. IMP3 is an oncofetal protein associated with aggressive and advanced tumors and is specifically expressed in malignant tumors but not found in benign tissues [PMID:22497850]. IMP3 forms a ternary complex with IMP4 and MPP10 (MPHOSPH10) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing[PMID: 12655004].

Protocols

Product Specific Protocols
WB protocol for IMP3 antibody 66247-1-Ig	Download protocol
IHC protocol for IMP3 antibody 66247-1-Ig	Download protocol
IF protocol for IMP3 antibody 66247-1-Ig	Download protocol