CoraLite®555-conjugated IMP3 Monoclonal antibody

IMP3 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1E7D6

Cat no : CL555-66247

Synonyms

MRPS4, IMP 3, C15orf12, BRMS2, 1E7D6

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Validation Data Gallery

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  FC experiment of HepG2 using CL555-66247

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human IMP3 (CL555-66247, Clone:1E7D6) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66247 targets IMP3 in FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IMP3 fusion protein Ag23602
• Full Name: IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
• Calculated Molecular Weight: 184 aa, 22 kDa
• GenBank Accession Number: BC020557
• Gene Symbol: IMP3
• Gene ID (NCBI): 55272
• RRID: AB_2919680
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

IMP3 is a member of the insulin-like growth factor (IGF-II) mRNA binding protein (IMP) family that consist of IMP1, IMP2, and IMP3. IMP3 is an oncofetal protein associated with aggressive and advanced tumors and is specifically expressed in malignant tumors but not found in benign tissues [PMID:22497850]. IMP3 forms a ternary complex with IMP4 and MPP10 (MPHOSPH10) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing[PMID: 12655004].

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Foxp3 / Transcription Factor Staining Buffer Kit

PF00011