IMP3 Monoclonal antibody, PBS Only

IMP3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E7D6

Cat no : 66247-1-PBS

Synonyms

MRPS4, IMP 3, C15orf12, BRMS2, 1E7D6

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Validation Data Gallery

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  WB analysis of HeLa using 66247-1-Ig (same clone as 66247-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66247-1-Ig (IMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66247-1-Ig (IMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

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  WB analysis of SKOV-3 using 66247-1-Ig (same clone as 66247-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66247-1-Ig (IMP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66247-1-Ig (same clone as 66247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66247-1-Ig (same clone as 66247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66247-1-Ig (same clone as 66247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66247-1-Ig (same clone as 66247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66247-1-Ig (IMP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (66247-1-Ig, Clone: 1E7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66247-1-Ig (same clone as 66247-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human IMP3 (66247-1-Ig, Clone:1E7D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66247-1-PBS in a different storage buffer formulation.

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Product Information

66247-1-PBS targets IMP3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IMP3 fusion protein Ag23602
• Full Name: IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
• Calculated Molecular Weight: 184 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC020557
• Gene Symbol: IMP3
• Gene ID (NCBI): 55272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IMP3 is a member of the insulin-like growth factor (IGF-II) mRNA binding protein (IMP) family that consist of IMP1, IMP2, and IMP3. IMP3 is an oncofetal protein associated with aggressive and advanced tumors and is specifically expressed in malignant tumors but not found in benign tissues [PMID:22497850]. IMP3 forms a ternary complex with IMP4 and MPP10 (MPHOSPH10) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing[PMID: 12655004].