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Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody

CD14 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

26IC

Cat no : 65616-1-MR

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 26IC

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Ant (65616-1-MR)

    FC experiment of human PBMCs using 65616-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody (65616-1-MR, Clone: 26IC) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Ant (65616-1-MR)

    FC experiment of human PBMCs using 65616-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody (65616-1-MR, Clone:26IC) (red) or Mouse IgG2a Isotype Control (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65616-1-MR targets CD14 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A
Full Name CD14 molecule
Calculated Molecular Weight 375 aa, 40 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
FC protocol for CD14 antibody 65616-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols