1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a RecAb (Biotin-65616, Clone:26IC) and CoraLite®488-conjugated streptavidin, or unstained cells. Cells were co-stained with APC Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a RecAb (Biotin-65616, Clone:26IC) and CoraLite®488-conjugated streptavidin, or unstained cells. Cells were co-stained with APC Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human PBMCs using Biotin-65616
1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a RecAb (Biotin-65616, Clone:26IC)(red) and CoraLite®488-conjugated streptavidin, or unstained cells(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a RecAb (Biotin-65616, Clone:26IC)(red) and CoraLite®488-conjugated streptavidin, or unstained cells(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
Biotin-65616 targets CD14 in FC applications and shows reactivity with human samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.
1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a RecAb (Biotin-65616, Clone:26IC) and CoraLite®488-conjugated streptavidin, or unstained cells. Cells were co-stained with APC Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human PBMCs using Biotin-65616
1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD14 (26IC) Mouse IgG2a RecAb (Biotin-65616, Clone:26IC)(red) and CoraLite®488-conjugated streptavidin, or unstained cells(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD14 Recombinant antibody
Catalog Number
Biotin-65616
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
