Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody, PBS Only

CD14 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

26IC

Cat no : 65616-1-PBS

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 26IC

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Validation Data Gallery

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  FC experiment of human PBMCs using 65616-1-MR (same clone as 65616-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody (65616-1-MR, Clone: 26IC) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 65616-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65616-1-MR (same clone as 65616-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody (65616-1-MR, Clone:26IC) (red) or Mouse IgG2a Isotype Control (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 65616-1-PBS in a different storage buffer formulation.

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Product Information

65616-1-PBS targets CD14 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD14 molecule
• Calculated Molecular Weight: 375 aa, 40 kDa
• GenBank Accession Number: BC010507
• Gene Symbol: CD14
• Gene ID (NCBI): 929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.