• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Mouse CD11c (N418)

CD11c Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

N418

Cat no : 65130-1-Ig

Synonyms

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of C57BL/6 mouse splenocytes using Anti-Mouse CD11c (N418) (65130-1-Ig)

    FC experiment of C57BL/6 mouse splenocytes using 65130-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with APC Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) and 0.5 ug Anti-Mouse CD11c (65130-1-Ig, Clone:N418) and FITC anti-Armenian Hamster IgG Antibody at dilution 1:100, or 0.5 ug Armenian Hamster IgG Isotype Control (PIP) and FITC anti-Armenian Hamster IgG Antibody at dilution 1:100. Cells were not fixed.

Tested Applications

Positive FC detected inC57BL/6 mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65130-1-Ig targets CD11c in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen Mouse spleen dendritic cells
Full Name integrin alpha X
GenBank Accession NumberBC167225
Gene Symbol CD11c
Gene ID (NCBI) 16411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Protocols

Product Specific Protocols
FC protocol for CD11c antibody 65130-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols