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JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10023-1-AP

Synonyms

MAPK8, JNK1A2, JNK1, JNK 2, JNK 1

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using JNK Polyclonal antibody (10023-1-AP)

    WB analysis of NIH/3T3 using 10023-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using JNK Polyclonal antibody (10023-1-AP)

    WB analysis of MCF-7 using 10023-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using JNK Polyclonal antibody (10023-1-AP)

    WB analysis of MCF-7 using 10023-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using JNK Polyclonal antibody (10023-1-AP)

    WB analysis of Jurkat using 10023-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using JNK Polyclonal antibody (10023-1-AP)

    WB analysis of HeLa using 10023-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using JNK Polyclonal antibody (10023-1-AP)

    WB analysis of RAW 264.7 using 10023-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using JNK Polyclonal antibody (10023-1-AP)

    WB analysis of HEK-293 using 10023-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using JNK Polyclonal antibody (10023-1-AP)

    WB analysis of SH-SY5Y using 10023-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using JNK Polyclonal antibody (10023-1-AP)

    WB analysis of RAW264.7 using 10023-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using JNK Polyclonal antibody (10023-1-AP)

    WB analysis of A431 using 10023-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using JNK Polyclonal antibody (10023-1-AP)

    IHC staining of human prostate cancer using 10023-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using JNK Polyclonal antibody (10023-1-AP)

    IHC staining of human prostate cancer using 10023-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using JNK Polyclonal antibody (10023-1-AP)

    IHC staining of human lung cancer using 10023-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using JNK Polyclonal antibody (10023-1-AP)

    IHC staining of human lung cancer using 10023-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using JNK Polyclonal antibody (10023-1-AP)

    IHC staining of mouse heart using 10023-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using JNK Polyclonal antibody (10023-1-AP)

    IHC staining of mouse heart using 10023-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using JNK Polyclonal antibody (10023-1-AP)

    IF Staining of HeLa using 10023-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JNK antibody (10023-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inNIH/3T3 cells, A431 cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, RAW 264.7 cells, RAW264.7, SH-SY5Y cells
Positive IHC detected inhuman prostate cancer tissue, human lung cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10023-1-AP targets JNK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JNK fusion protein Ag0019
Full Name mitogen-activated protein kinase 8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 40-45 kDa, 50-55 kDa
GenBank Accession NumberNM_138982
Gene Symbol JNK
Gene ID (NCBI) 5599
RRIDAB_2281669
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Protocols

Product Specific Protocols
WB protocol for JNK antibody 10023-1-APDownload protocol
IHC protocol for JNK antibody 10023-1-APDownload protocol
IF protocol for JNK antibody 10023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Pineal Res

Melatonin reprogramming of gut microbiota improves lipid dysmetabolism in high fat diet-fed mice.

Authors - Jie Yin
View Article
human

Front Oncol

Upregulation of OTUD7B (Cezanne) Promotes Tumor Progression via AKT/VEGF Pathway in Lung Squamous Carcinoma and Adenocarcinoma.

Authors - Dan-Dan Lin
View Article
humanWB

Neuropharmacology

TRPC6-mediated ERK1/2 phosphorylation prevents dentate granule cell degeneration via inhibiting mitochondrial elongation.

Authors - Ah-Reum Ko
View Article
ratWB

Ecotoxicol Environ Saf

PM2.5 exposure induces reproductive injury through IRE1/JNK/autophagy signaling in male rats.

Authors - Yang Yang
View Article
mouseWB

J Biol Chem

Inactivation of mouse transmembrane prolyl 4-hydroxylase increases blood brain barrier permeability and ischemia-induced cerebral neuroinflammation.

Authors - Nadiya Byts
View Article
humanWB

Front Pharmacol

BCATc inhibitor 2 ameliorated mitochondrial dysfunction and apoptosis in oleic acid-induced non-alcoholic fatty liver disease model

Authors - Zhuo Lu
View Article