c-Fos Polyclonal antibody

• Featured Product
• KD/KO Validated

c-Fos Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

30

Reviews (2)

Cat no : 26192-1-AP

Synonyms

AP 1, C FOS, Cellular oncogene fos, c-Fos, FOS, G0S7, Proto oncogene c Fos

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Validation Data Gallery

• 

  WB analysis of RAW 264.7 using 26192-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26192-1-AP (c-Fos antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26192-1-AP (c-Fos antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26192-1-AP (c-FOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26192-1-AP (c-FOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26192-1-AP (c-FOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 10 publications below
• IF: See 10 publications below

Product Information

The immunogen of 26192-1-AP is c-Fos Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: c-Fos fusion protein Ag24340
• Full Name: FOS
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC004490
• Gene Symbol: c-Fos
• Gene ID (NCBI): 2353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Fos, also named as FOS and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. c-Fos is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, c-Fos undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the c-Fos protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the c-FOS protein becomes nuclear. c-Fos is a very short-lived intracellular protein, which is very easy to degrade. The calculated molecular weight of c-Fos is 40 kDa, but Phosphorylated c-Fos protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-Fos is an indirect marker of neuronal activity because c-Fos is often expressed when neurons fire action potentials. Upregulation of c-Fos mRNA in a neuron indicates recent activity.

Publications

Species	Application	Title
mouse	IHC, IF	Nature; Mechanisms of stretch-mediated skin expansion at single-cell resolution.; Authors - Mariaceleste Aragona
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
	IF	Sci Adv; GPR39 regulated spinal glycinergic inhibition and mechanical inflammatory pain; Authors - Hu-Hu Bai
mouse	IHC	Nat Commun; Ketamine activates adult-born immature granule neurons to rapidly alleviate depression-like behaviors in mice.; Authors - Radhika Rawat
mouse	IHC	Biol Psychiatry; Fighting Females: Neural and Behavioral Consequences of Social Defeat Stress in Female Mice.; Authors - Emily L Newman
human	IHC	Nat Commun; Excitatory VTA to DH projections provide a valence signal to memory circuits.; Authors - Yuan Han

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Matthias (Verified Customer) (09-10-2019)

Good Antibody, very specific.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 2000
• Cell Tissue Type: Brain tissue

c-Fos Antibody Immunohistochemistry,Immunofluorescence, validation (2000 dilution) in Brain tissue (Cat no:26192-1-AP)

FH

Elif (Verified Customer) (06-21-2019)

It works better with 2 overnight incubation in 4 degrees.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Dentate gyrus

c-Fos Antibody Immunohistochemistry, validation (1:500 dilution) in Dentate gyrus (Cat no:26192-1-AP)