Anticorps Polyclonal de lapin anti-WFS1

• Phare
• Validé par KD/KO

WFS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, coIP, ELISA

Conjugaison

Non conjugué

Publications

72

Avis (1)

N° de cat : 11558-1-AP

Synonymes

WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 11558-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11558-1-AP (WFS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11558-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11558-1-AP (WFS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 11558-1-AP

  IP result of anti-WFS1 (IP:11558-1-AP, 4ug; Detection:11558-1-AP 1:400) with SH-SY5Y cells lysate 770 ug.

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  IHC staining of rat brain using 11558-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11558-1-AP (WFS1 antibody) at dilution of 1:400 (under 40x lens)..

• 

  IF Staining of mouse brain using 11558-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11558-1-AP (WFS1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66564-1-Ig (Tbr1 antibody) and and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of rat brain using 11558-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using WFS1 antibody (11558-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 11558-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11558-1-AP (WFS1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules HEK-293
• Résultats positifs en IP: cellules SH-SY5Y,
• Résultats positifs en IHC: tissu cérébral de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 17 publications below
• IF: See 37 publications below
• CoIP: See 1 publications below

Informations sur le produit

11558-1-AP cible WFS1 dans les applications de WB, IP, IF, IHC, coIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WFS1 Protéine recombinante Ag2114
• Nom complet: Wolfram syndrome 1 (wolframin)
• Masse moléculaire calculée: 890 aa, 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC030130
• Symbole du gène: WFS1
• Identification du gène (NCBI): 7466
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.

Publications

Species	Application	Title
mouse	IF	Science; Monosomes actively translate synaptic mRNAs in neuronal processes.; Authors - Anne Biever
mouse	IF	Cell; A Hippocampus-Accumbens Tripartite Neuronal Motif Guides Appetitive Memory in Space.; Authors - Stéphanie Trouche
mouse	IF	Nature; The hippocampal CA2 region is essential for social memory.; Authors - Frederick L Hitti
mouse	IF	Science; Island cells control temporal association memory.; Authors - Takashi Kitamura
mouse	IF	Cell; A Map-like Micro-Organization of Grid Cells in the Medial Entorhinal Cortex.; Authors - Yi Gu
mouse	IF	Cell; Distinct Neural Circuits for the Formation and Retrieval of Episodic Memories.; Authors - Dheeraj S Roy

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lana (Verified Customer) (05-29-2020)

SDS-PAGE: 15 ug/ul RIPA lysate of whole brain tissue or mitochondrial fraction. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000Lines of WB: 1 – protein ladder, 2 – whole brain tissue lysate, 3 – mitochondrial fraction lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Whole brain tissue (human), mitochondrial fraction from brain tissue (human).

WFS1 Antibody Western Blot validation (1:1000 dilution) in Whole brain tissue (human), mitochondrial fraction from brain tissue (human). (Cat no:11558-1-AP)

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