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SLC30A8/ZNT8-Specific Polyclonal antibody
SLC30A8/ZNT8-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 16169-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse pancreas tissue |
| Positive IHC detected in | human pancreas tissue, human pancreas cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
16169-1-AP targets SLC30A8/ZNT8-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, Yellow catfish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | solute carrier family 30 (zinc transporter), member 8 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | NM_173851 |
| Gene Symbol | SLC30A8 |
| Gene ID (NCBI) | 169026 |
| RRID | AB_10733889 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC30A8 (also named as ZNT8) encods the secretory granule zinc transporter, permiting cellular efflux of zinc. SLC30A8 was recently defined as a autoantigen candidates of type 2 diabetes, although the molecular mechanism through which this phenotype is manifest is incompletely understood.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC30A8/ZNT8-Specific antibody 16169-1-AP | Download protocol |
| IHC protocol for SLC30A8/ZNT8-Specific antibody 16169-1-AP | Download protocol |
| IF protocol for SLC30A8/ZNT8-Specific antibody 16169-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem Lipid-tuned Zinc Transport Activity of Human ZnT8 Correlates with Risk for Type-2 Diabetes. | ||
J Biol Chem A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells. | ||
J Biol Chem Highly specific monoclonal antibodies for allosteric inhibition and immunodetection of the human pancreatic zinc transporter ZnT8. | ||
J Endocrinol Lack of ZnT8 protects pancreatic islets from hypoxia- and cytokine induced cell death. | ||
Sci Rep A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells. |
