Kir6.2 Polyclonal antibody

• 

  WB analysis of rat heart using 16920-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (Kir6.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 16920-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (Kir6.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16920-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16920-1-AP (KCNJ11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16920-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (KCNJ11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Kir6.2 Polyclonal Antibody for WB, ELISA

Cat No. 16920-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

BIR, HHF2, IKATP, KCNJ11, KIR6.2, PHHI, TNDM3

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Tested Applications

• Positive WB detected in: rat heart tissue, HepG2 cells, human heart tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16920-1-AP targets Kir6.2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10262

  Product name: Recombinant human Kir6.2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 169-390 aa of BC064497

  Sequence: MKTAQAHRRAETLIFSKHAVIALRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPEGEVVPLHQVDIPMENGVGGNSIFLVAPLIIYHVIDANSPLYDLAPSDLHHHQDLEIIVILEGVVETTGITTQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTIKVPTPLCTARQLDEDHSLLEALTLASARGPLRKRSVPMAKAKPKFSISPDSLS

• Full Name: potassium inwardly-rectifying channel, subfamily J, member 11
• Calculated Molecular Weight: 390 aa, 44 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC064497
• Gene Symbol: Kir6.2
• Gene ID (NCBI): 3767
• RRID: AB_2130461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14654
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kir6.2 (also known as BIR or IKATP), encoded by the KCNJ11 gene, is the pore-forming unit of the ATP-sensitive K+ channel, an inward-rectifier potassium ion channel. Kir6.2 is controlled by G-proteins and is found associated with the sulfonylurea receptor (SUR) to constitute the ATP-sensitive K+ channel. The KCNJ11 gene is located at 11p15.1 and has no intron. Mutations in KCNJ11 are a cause of familial PHHI, an autosomal recessive disorder characterized by unregulated ins secretion. Defects in KCNJ11 may also contribute to autosomal dominant non-ins-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Int Immunopharmacol; Nicorandil restores endothelial cell Kir6.2 expression to alleviate neuropathic pain in mice after chronic constriction injury; Authors - Minghao Du
rat	WB	Sci Rep; Glucocorticoid receptor-NECAB1 axis can negatively regulate insulin secretion in pancreatic β-cells; Authors - Haruhide Udagawa
		Life Sci; KATP channels as ROS-dependent modulator of neurotransmitter release at the neuromuscular junctions; Authors - Andrei N Tsentsevitsky