TCF4/TCF7L2 Polyclonal antibody

TCF4/TCF7L2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 13838-1-AP

Synonyms

HMG box transcription factor 4, hTCF 4, T cell factor 4, TCF 4, TCF4, TCF4/TCF7L2, TCF7L2, Transcription factor 7 like 2

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Validation Data Gallery

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  WB analysis using 13838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF4/TCF7L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13838-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 13838-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13838-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13838-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13838-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13838-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 13838-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13838-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13838-1-AP

  IP result of anti-TCF4/TCF7L2 (IP:13838-1-AP, 4ug; Detection:13838-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, human brain tissue, human heart tissue, HepG2 cells, mouse kidney tissue, mouse heart tissue, mouse placenta tissue, rat liver tissue, HEK-293 cells
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Product Information

13838-1-AP targets TCF4/TCF7L2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCF4/TCF7L2 fusion protein Ag4786
• Full Name: transcription factor 7-like 2 (T-cell specific, HMG-box)
• Calculated Molecular Weight: 619 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC032656
• Gene Symbol: TCF4
• Gene ID (NCBI): 6934
• RRID: AB_2199812
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal; Authors - Jing Wang
human	WB	Oncogene; The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.; Authors - Kai Li
human	WB	Sci Rep; TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.; Authors - Fengqin Dong
human	WB,IF,CoIP	Front Cell Dev Biol; Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin.; Authors - Nanlan Yu
human	WB	Mol Cancer Res; Wnt/β-Catenin Signaling Axis Is Required for TFEB-Mediated Gastric Cancer Metastasis and Epithelial-Mesenchymal Transition.; Authors - Shuxuan Li
human	WB	J Biol Chem; HBP1-mediated regulation of p21 through Mdm2/p53 and TCF4/EZH2 pathways and its impact on cell senescence and tumorigenesis.; Authors - Yifan Chen