Anticorps Monoclonal anti-Vinculin
Vinculin Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, porc, rat, souris et plus (2)
Applications
WB, IHC, IF, FC, CoIP, ELISA
Conjugaison
Non conjugué
119
CloneNo.
2B5A7
N° de cat : 66305-1-Ig
Synonymes
"Vinculin Antibodies" Comparison
View side-by-side comparison of Vinculin antibodies from other vendors to find the one that best suits your research needs.
Applications testées
| Résultats positifs en WB | cellules HepG2, cellules C6, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules HuH-7, cellules L02, cellules LNCaP, cellules MG U-87, cellules NIH/3T3, cellules U-251, tissu cardiaque de porc |
| Résultats positifs en IHC | tissu de cancer du sein humain, tissu de cancer de la prostate humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | cellules HeLa |
| Résultats positifs en cytométrie | cellules HeLa |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochimie (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 89 publications below |
| IHC | See 2 publications below |
| IF | See 25 publications below |
| CoIP | See 1 publications below |
Informations sur le produit
66305-1-Ig cible Vinculin dans les applications de WB, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris
| Réactivité | Humain, porc, rat, souris |
| Réactivité citée | rat, Humain, poisson-zèbre, porc, souris, Hamster |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Vinculin Protéine recombinante Ag24946 |
| Nom complet | vinculin |
| Masse moléculaire calculée | 1133 aa, 124 kDa |
| Poids moléculaire observé | 117 kDa |
| Numéro d’acquisition GenBank | BC039174 |
| Symbole du gène | VCL |
| Identification du gène (NCBI) | 7414 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for Vinculin antibody 66305-1-Ig | Download protocol |
| IHC protocol for Vinculin antibody 66305-1-Ig | Download protocol |
| IF protocol for Vinculin antibody 66305-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Crit Care Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein. | ||
Circ. Res. Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm. | ||
Cell Death Differ Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis. | ||
Cell Death Differ Loss of RBMS1 promotes anti-tumor immunity through enabling PD-L1 checkpoint blockade in triple-negative breast cancer. | ||
ACS Appl Mater Interfaces Oscillating Magnetic Field Regulates Cell Adherence and Endothelialization Based on Magnetic Nanoparticle-Modified Bacterial Cellulose. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Parijat (Verified Customer) (08-21-2023) | Works well as loading control
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FH Udesh (Verified Customer) (08-16-2023) | Worked well in WB at 1:3000 and IF at 1:100
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FH Mohamad (Verified Customer) (07-03-2023) | Very good antibody
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FH Priya (Verified Customer) (01-17-2023) | I have used for human cardiomyocytes, mouse skin and liver tissues
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FH Macarena (Verified Customer) (10-07-2022) | excellent results.
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FH Jonas (Verified Customer) (07-29-2022) | This is my go to loading control stain to validate evenly loaded lanes Binding could be stronger but by using 1:500 dilution, a reliable staining can be achieved
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FH Charlotte (Verified Customer) (07-26-2022) | Very good antibody. Very specific, super fast to reveal. Here we see Gli1 (160 kDa) because it is a mouse antibody too.
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FH AKIMASA (Verified Customer) (04-28-2021) | I could get the good quality band!
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FH Chun (Verified Customer) (09-07-2020) | This is a fairly good antibody for immunoblotting.
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FH Huai-Chin (Verified Customer) (06-08-2019) | Serving as a loading control, this antibody is not that good compare to other. Still work to some extent.
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