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FAK Polyclonal antibody
FAK Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12636-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF7 cells, A431 cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human gliomas tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HUVEC cells, A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 49 publications below |
IHC | See 3 publications below |
IF | See 3 publications below |
Product Information
12636-1-AP targets FAK in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FAK fusion protein Ag3331 |
Full Name | PTK2 protein tyrosine kinase 2 |
Calculated molecular weight | 1052 aa, 119 kDa |
Observed molecular weight | 110 kDa |
GenBank accession number | BC028733 |
Gene symbol | PTK2 |
Gene ID (NCBI) | 5747 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for FAK antibody 12636-1-AP | Download protocol |
IHC protocol for FAK antibody 12636-1-AP | Download protocol |
IF protocol for FAK antibody 12636-1-AP | Download protocol |
IP protocol for FAK antibody 12636-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Res Retinoic acid-related orphan receptor C regulates proliferation, glycolysis, and chemoresistance via the PD-L1/ITGB6/STAT3 signaling axis in bladder cancer. | ||
Cell Death Dis BK ablation attenuates osteoblast bone formation via integrin pathway.
| ||
Am J Transl Res GRHL2 suppresses tumor metastasis via regulation of transcriptional activity of RhoG in non-small cell lung cancer. | ||
Int J Cardiol Mesoderm/mesenchyme homeobox gene l promotes vascular smooth muscle cell phenotypic modulation and vascular remodeling. | ||
Biochim Biophys Acta Mol Basis Dis Deficiency in augmenter of liver regeneration accelerates liver fibrosis by promoting migration of hepatic stellate cell. | ||
Cell Death Discov A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Nathalie (Verified Customer) (07-12-2022) | Good for ImmunoHistochemistry
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