FAK Polyclonal antibody

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  WB analysis using 12636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12636-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12636-1-AP

  IP result of anti-FAK (IP:12636-1-AP, 4ug; Detection:12636-1-AP 1:30000) with HeLa cells lysate 1600 ug.

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  IHC staining of human gliomas using 12636-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12636-1-AP (FAK antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 12636-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12636-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 12636-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FAK antibody (12636-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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  IF Staining of HUVEC using 12636-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using FAK antibody (12636-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• Featured Product
• KD/KO Validated

FAK Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12636-1-AP

Publications(109)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

PTK2, p125FAK, Focal adhesion kinase-related nonkinase, Focal adhesion kinase 1, FAK1

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Tested Applications

• Positive WB detected in: A431 cells, NIH/3T3 cells, HEK-293 cells, HUVEC cells, HeLa cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 96 publications below
• IHC: See 4 publications below
• IF: See 13 publications below
• CoIP: See 3 publications below

Product Information

12636-1-AP targets FAK in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAK fusion protein Ag3331
• Full Name: PTK2 protein tyrosine kinase 2
• Calculated Molecular Weight: 1052 aa, 119 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC028733
• Gene Symbol: FAK
• Gene ID (NCBI): 5747
• RRID: AB_2173668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05397
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Cell Biol; Gain of miR-151 on chromosome 8q24.3 facilitates tumour cell migration and spreading through downregulating RhoGDIA.; Authors - Ding Jie J
human	IF	Small; A Spatiotemporal Controllable Biomimetic Skin for Accelerating Wound Repair; Authors - Yuewei Chen
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
mouse	WB	J Hazard Mater; Polystyrene microplastics induced male reproductive toxicity in mice.; Authors - Haibo Jin
human	WB	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
human	WB	Oncogene; Circadian regulator NR1D2 regulates glioblastoma cell proliferation and motility.; Authors - Min Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nathalie (Verified Customer) (07-12-2022)

Good for ImmunoHistochemistry

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: Human Skin