FAK Polyclonal antibody

• Featured Product
• KD/KO Validated

FAK Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

55

Reviews (1)

Cat no : 12636-1-AP

Synonyms

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 12636-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12636-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12636-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12636-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12636-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12636-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12636-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12636-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12636-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12636-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12636-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12636-1-AP

  IP Result of anti-FAK (IP:12636-1-AP, 3ug; Detection:12636-1-AP 1:2000) with mouse brain tissue lysate 8000ug.

• 

  IHC staining of human gliomas using 12636-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12636-1-AP (FAK antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 12636-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12636-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 12636-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using FAK antibody (12636-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  IF Staining of A549 using 12636-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FAK antibody (12636-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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Tested Applications

• Positive WB detected in: MCF7 cells, A431 cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HUVEC cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 49 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

12636-1-AP targets FAK in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAK fusion protein Ag3331
• Full Name: PTK2 protein tyrosine kinase 2
• Calculated molecular weight: 1052 aa, 119 kDa
• Observed molecular weight: 110 kDa
• GenBank accession number: BC028733
• Gene symbol: PTK2
• Gene ID (NCBI): 5747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

Publications

Species	Application	Title
human	WB	Cancer Res; Retinoic acid-related orphan receptor C regulates proliferation, glycolysis, and chemoresistance via the PD-L1/ITGB6/STAT3 signaling axis in bladder cancer.; Authors - Dalong Cao
mouse	WB	Cell Death Dis; BK ablation attenuates osteoblast bone formation via integrin pathway.; Authors - Yinhang Wang ; KD Validated
human	WB	Am J Transl Res; GRHL2 suppresses tumor metastasis via regulation of transcriptional activity of RhoG in non-small cell lung cancer.; Authors - Xiang Pan
rat	WB	Int J Cardiol; Mesoderm/mesenchyme homeobox gene l promotes vascular smooth muscle cell phenotypic modulation and vascular remodeling.; Authors - Bing Wu
		Biochim Biophys Acta Mol Basis Dis; Deficiency in augmenter of liver regeneration accelerates liver fibrosis by promoting migration of hepatic stellate cell.; Authors - Wei-Lun Ai
human	WB	Cell Death Discov; A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein.; Authors - Pengyun Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nathalie (Verified Customer) (07-12-2022)

Good for ImmunoHistochemistry

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: Human Skin

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