|Positive WB detected in||MCF7 cells, A431 cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12636-1-AP targets FAK in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FAK fusion protein Ag3331|
|Full Name||PTK2 protein tyrosine kinase 2|
|Calculated molecular weight||1052 aa, 119 kDa|
|Observed molecular weight||110 kDa|
|GenBank accession number||BC028733|
|Gene ID (NCBI)||5747|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.
|Product Specific Protocols|
|WB protocol for FAK antibody 12636-1-AP||Download protocol|
|IHC protocol for FAK antibody 12636-1-AP||Download protocol|
|IF protocol for FAK antibody 12636-1-AP||Download protocol|
|IP protocol for FAK antibody 12636-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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