Anticorps Polyclonal de lapin anti-Vinculin

Vinculin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

59

Avis (5)

N° de cat : 26520-1-AP

Synonymes

CMD1W, Metavinculin, MVCL, VCL, Vinculin

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Galerie de données de validation

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  WB analysis using 26520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26520-1-AP (Vinculin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 26520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26520-1-AP (Vinculin antibody) at dilution of 1:600 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-phalloidine stains F-actin (red).

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  IF Staining of HepG2 using 26520-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 26520-1-AP (Vinculin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 26520-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Vinculin (26520-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MDCK, cellules NIH/3T3, cellules PC-12, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de côlon de souris, tissu de cancer de la prostate humain, tissu de cancer du sein humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:300-1:1200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 42 publications below
• IF: See 24 publications below

Informations sur le produit

26520-1-AP cible Vinculin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Vinculin Protéine recombinante Ag24942
• Nom complet: vinculin
• Masse moléculaire calculée: 1133 aa, 124 kDa
• Poids moléculaire observé: 117 kDa
• Numéro d’acquisition GenBank: BC039174
• Symbole du gène: VCL
• Identification du gène (NCBI): 7414
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Publications

Species	Application	Title
human	WB,IF	ACS Nano; Polyethylenimine-Modified Mesoporous Silica Nanoparticles Induce a Survival Mechanism in Vascular Endothelial Cells via Microvesicle-Mediated Autophagosome Release; Authors - Lu Zhang
mouse	IF	Adv Sci (Weinh); Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min.; Authors - Mu Li
rabbit	WB,IF	Adv Sci (Weinh); Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing; Authors - Yingni Xu
human	WB	Nat Commun; Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.; Authors - Kai Yuan
human	IF	Small; Versatile Protein Coronation Approach with Multiple Depleted Serum for Creating Biocompatible, Precision Nanomedicine.; Authors - Chan Wang
human	WB	J Exp Clin Cancer Res; RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization; Authors - Shuang Tao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (12-04-2023)

Very good antibody for detection of vinculin by WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HEKs / HUVEC

Vinculin Antibody Western Blot validation (1 in 1000 dilution) in HEKs / HUVEC (Cat no:26520-1-AP)

FH

Verdiana (Verified Customer) (11-03-2022)

Band at about 140kDa. Great antibody! Bands were visible just after 0.5sec of exposure! Clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 in TBST 5% Milk
• Cell Tissue Type: U2OS

Vinculin Antibody Western Blot validation (1:2000 in TBST 5% Milk dilution) in U2OS (Cat no:26520-1-AP)

FH

Bettina (Verified Customer) (08-03-2022)

Antibody worked well!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse pancreatic cancer cell line

Vinculin Antibody Western Blot validation (1:1000 dilution) in Mouse pancreatic cancer cell line (Cat no:26520-1-AP)

FH

Silvia (Verified Customer) (02-14-2022)

Good sharp clear band in western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000

FH

Bhuvanasundar (Verified Customer) (10-12-2021)

Immunofluorescence staining with this antibody worked very well.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Epithelial cell

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