Anticorps Polyclonal de lapin anti-VEGFA

• Phare
• Validé par KD/KO

VEGFA Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

712

Avis (3)

N° de cat : 19003-1-AP

Synonymes

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Galerie de données de validation

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  WB analysis using 19003-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19003-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGFA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19003-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 19003-1-AP

  IP result of anti-VEGFA (IP:19003-1-AP, 4ug; Detection:19003-1-AP 1:3000) with Jurkat cells lysate 1560 ug.

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  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19003-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VEGFA antibody (19003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 19003-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human VEGFA (19003-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain, tissu hépatique de souris, tissu pulmonaire de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules NIH/3T3
• Résultats positifs en cytométrie: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 32 publications below
• IHC: See 223 publications below
• IF: See 75 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

19003-1-AP cible VEGFA dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Chèvre, Humain, Lapin, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VEGFA Protéine recombinante Ag13500
• Nom complet: vascular endothelial growth factor A
• Masse moléculaire calculée: 412 aa, 46 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC065522
• Symbole du gène: VEGFA
• Identification du gène (NCBI): 7422
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VEGFA, also named VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

Publications

Species	Application	Title
mouse	IHC	Nat Nanotechnol; Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.; Authors - Xuefei Lu ; KD Validated
human		Bioact Mater; A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds; Authors - Shunlai Shang
mouse,human		ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao
mouse	IHC	Biomaterials; A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration; Authors - Congcong Yu
mouse		Light Sci Appl; Microglia modulation with 1070-nm light attenuates Aβ burden and cognitive impairment in Alzheimer's disease mouse model.; Authors - Lechan Tao
human	IP	J Clin Invest; Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema.; Authors - Akrit Sodhi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Angie (Verified Customer) (07-24-2024)

WB result of VEGFA antibody (used at 1:5000) incubated at room temperature for 1.5 hours. Two stronger bands between 35-50 kDa were observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: A549

VEGFA Antibody Western Blot validation (1:5000 dilution) in A549 (Cat no:19003-1-AP)

FH

Alexandru (Verified Customer) (11-21-2023)

Great antibody with clear and specific signal, would buy again!

• Applications: Western Blot
• Cell Tissue Type: Mouse Ventricle

FH

Fiona (Verified Customer) (07-16-2019)

Good quality antibody and good value for money. Would purchase again.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

VEGFA Antibody Western Blot, validation (1:500 dilution) in ARPE-19 (Cat no:19003-1-AP)

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