ANGPTL4 Polyclonal antibody

• Featured Product
• KD/KO Validated

ANGPTL4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

31

Reviews (1)

Cat no : 18374-1-AP

Synonyms

ANGPTL2, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Angiopoietin related protein 4, Angiopoietin like protein 4

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Validation Data Gallery

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  WB analysis using 18374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18374-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18374-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18374-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 18374-1-AP

  IP result of anti-ANGPTL4 (IP:18374-1-AP, 4ug; Detection:18374-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human kidney using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human ovary using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

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  IF Staining of NIH/3T3 using 18374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HT-29 using 18374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 18374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse kidney tissue, HEK-293 cells, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human kidney tissue, human oesophagus cancer tissue, human heart tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells, HT-29 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 20 publications below
• IHC: See 13 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

18374-1-AP targets ANGPTL4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANGPTL4 fusion protein Ag13237
• Full Name: angiopoietin-like 4
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC023647
• Gene Symbol: ANGPTL4
• Gene ID (NCBI): 51129
• RRID: AB_2878539
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.

Publications

Species	Application	Title
		Nat Metab; Resistant starch intake facilitates weight loss in humans by reshaping the gut microbiota; Authors - Huating Li
human	IP	J Clin Invest; Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema.; Authors - Akrit Sodhi
mouse	WB	EMBO J; LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.; Authors - Yanying Wang
mouse	IF	J Pathol; ANGPTL4-αvβ3 integrin interaction counteracts hypoxia-induced vascular permeability by modulating Src signalling downstream of VEGFR2.; Authors - Elisa Gomez Perdiguero
human	IHC	Breast Cancer Res; An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-beta in human breast cancer.; Authors - Misako Sato
human	IHC,WB	J Cell Mol Med; Decreased ANGPTL4 impairs endometrial angiogenesis during peri-implantation period in patients with recurrent implantation failure.; Authors - Mingyang Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Niklas (Verified Customer) (11-28-2022)

bright and specific staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:350
• Cell Tissue Type: human fibroblasts