Anticorps Polyclonal de lapin anti-p63

• Phare
• Validé par KD/KO

p63 Polyclonal Antibody for WB, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

32

Avis (2)

N° de cat : 12143-1-AP

Synonymes

TP63, tumor protein p63, Tumor protein 63, Transformation-related protein 63, TP 63

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Galerie de données de validation

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  WB analysis using 12143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12143-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Apoptosised HeLa using 12143-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 12143-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12143-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12143-1-AP (TP63 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IP experiment of A431 using 12143-1-AP

  IP result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:1000) with A431 cells lysate 1200 ug.

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  IP experiment of A431 using 12143-1-AP

  IP result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:300) with A431 cells lysate 1200ug.

• 

  IHC staining of human tonsillitis using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12143-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung cancer using 12143-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using p63 antibody (12143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lung cancer using 12143-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using p63 antibody (12143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 12143-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using p63 antibody (12143-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• 

  FC experiment of A431 using 12143-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human p63 (12143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HaCaT, cellules HeLa apoptosées, cellules PC-12, cellules U-937, tissu cardiaque de souris, tissu cardiaque humain, tissu de thymus de souris
• Résultats positifs en IP: cellules A431,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du poumon humain,
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

12143-1-AP cible p63 dans les applications de WB, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: p63 Protéine recombinante Ag2791
• Nom complet: tumor protein p63
• Masse moléculaire calculée: 680 aa, 77 kDa
• Poids moléculaire observé: 63-68 kDa
• Numéro d’acquisition GenBank: BC039815
• Symbole du gène: TP63
• Identification du gène (NCBI): 8626
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.

Publications

Species	Application	Title
mouse		Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB,IP,ChIP	Nucleic Acids Res; Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma.; Authors - Yongsheng Huang ; KD Validated
human	IF	Cell Rep; A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma; Authors - Yue Li
mouse		Oncogene; TP53, CDKN2A/P16, and NFE2L2/NRF2 regulate the incidence of pure- and combined-small cell lung cancer in mice.; Authors - Samera H Hamad
human	IF	Elife; Chloride channels regulate differentiation and barrier functions of the mammalian airway.; Authors - Mu He
human		Oncogene; Circulating IGF-1 promotes prostate adenocarcinoma via FOXO3A/BIM signaling in a double-transgenic mouse model.; Authors - Shuang Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Kui (Verified Customer) (09-19-2019)

Best P63 Ab I can find so far.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Esophagus

p63 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse Esophagus (Cat no:12143-1-AP)

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