YAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

YAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

293

Reviews (8)

Cat no : 13584-1-AP

Synonyms

Yes-associated protein 1, yes associated protein 1, YAP, Transcriptional coactivator YAP1, Protein yorkie homolog

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Validation Data Gallery

• 

  WB analysis using 13584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13584-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13584-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 13584-1-AP

  IP result of anti-YAP1 (IP:13584-1-AP, 3ug; Detection:13584-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.

• 

  IHC staining of human liver cancer using 13584-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13584-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13584-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13584-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13584-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13584-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13584-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13584-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13584-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13584-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13584-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung cancer using 13584-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using YAP1 antibody (13584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lung cancer using 13584-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using YAP1 antibody (13584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 13584-1-AP

  Immunofluorescent analysis of HepG2 cells using 13584-1-AP (YAP1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 13584-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using YAP1 antibody (13584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, BGC-823 cells, MCF-7 cells, HepG2 cells, SGC-7901 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue, human ovary tumor tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human lung cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 35 publications below
• WB: See 234 publications below
• IHC: See 68 publications below
• IF: See 103 publications below
• IP: See 14 publications below
• CoIP: See 19 publications below
• ChIP: See 1 publications below

Product Information

13584-1-AP targets YAP1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YAP1 fusion protein Ag4510
• Full Name: Yes-associated protein 1, 65kDa
• Calculated Molecular Weight: 504 aa, 54 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: BC038235
• Gene Symbol: YAP1
• Gene ID (NCBI): 10413
• RRID: AB_2218915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers (PMID: 31613226, 32488048, 33520338). Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (PMID: 20048001).This antibody is a rabbit polyclonal antibody. The calcualted molecular weight of YAP1 is 54 kDa, but routinely observed at 65-75 kDa by Western Blot (PMID: 28230103, 33264286, 36255405).

Publications

Species	Application	Title
mouse	IF	Nature; Mechanisms of stretch-mediated skin expansion at single-cell resolution.; Authors - Mariaceleste Aragona
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
	IF	Nature; Fat1 deletion promotes hybrid EMT state, tumour stemness and metastasis.; Authors - Ievgenia Pastushenko
mouse	WB	Nature; YAP/TAZ activity in stromal cells prevents ageing by controlling cGAS-STING.; Authors - Hanna Lucie Sladitschek-Martens
human	IF	Nature; The SWI/SNF complex is a mechanoregulated inhibitor of YAP and TAZ.; Authors - Lei Chang
mouse	IF	Nat Mater; Reprogramming normal cells into tumour precursors requires ECM stiffness and oncogene-mediated changes of cell mechanical properties.; Authors - Tito Panciera

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Siddharth (Verified Customer) (08-04-2023)

Great for IF. Detects both Cytoplasmic and Nuclear YAP1.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: hMSC cell line

YAP1 Antibody Immunofluorescence validation (1:100 dilution) in hMSC cell line (Cat no:13584-1-AP)

FH

Chiara (Verified Customer) (09-15-2022)

In RH4 cells I observe by Western blot 4 bands; two bands around 70 KDa correspond to YAP, while a single band around 55 KDa corresponds to TAZ protein as proved by a RNA interference experiment.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RH4 Rhabdomyosarcoma cell line

YAP1 Antibody Western Blot validation (1:1000 dilution) in RH4 Rhabdomyosarcoma cell line (Cat no:13584-1-AP)

FH

María (Verified Customer) (02-08-2022)

Works for WB (1:1000).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Arianna (Verified Customer) (03-01-2019)

Genetically validated on YAP-null liver sections.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Liver tissue

FH

Joshua (Verified Customer) (12-20-2018)

Cells were fixed in 4% paraformaldehyde and stained overnight at 4C. Cells were counterstained with DAPI and phalloidin. Stain was mix of nuclear, cytosolic, and junctional.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MDCK Canine Kidney Epithelial

YAP1 Antibody Immunofluorescence, validation (1:500 dilution) in MDCK Canine Kidney Epithelial (Cat no:13584-1-AP)

FH

Joshua (Verified Customer) (12-20-2018)

Cells were fixed in 4% paraformaldehyde and stained overnight at 4C. Cells were counterstained with phalliodin and DAPI. Staining is mix of nuclear, junctional, and cytosolic.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MDCK Canine Kidney Epithelial

YAP1 Antibody Immunofluorescence, validation (1:500 dilution) in MDCK Canine Kidney Epithelial (Cat no:13584-1-AP)

FH

Juan Pablo (Verified Customer) (11-29-2018)

CGN treated with Shh (1ug/ml) for 48hs to induced proliferation, I see a nice induction of YAP1I get a nice clean band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Culture of Granule cell neurons from P7 rat pups

YAP1 Antibody Western Blot, validation (1:1000 dilution) in Culture of Granule cell neurons from P7 rat pups (Cat no:13584-1-AP)

FH

kk (Verified Customer) (11-21-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human PANC1 cells

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