GLUT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GLUT1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ChIP, ELISA

Conjugate

Unconjugated

Publications

282

Reviews (6)

Cat no : 21829-1-AP

Synonyms

SLC2A1, SLC2A1,GLUT1, GLUT-1

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Validation Data Gallery

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  WB analysis using 21829-1-AP

  HT-29 cell lysates (boiled or unboiled) were subjected to SDS PAGE followed by western blot with 21829-1-AP (GLUT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21829-1-AP

  Mouse colon lysates (boiled or unboiled) were subjected to SDS PAGE followed by western blot with 21829-1-AP (GLUT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21829-1-AP

  Mouse brain lysates (boiled or unboiled) were subjected to SDS PAGE followed by western blot with 21829-1-AP (GLUT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 21829-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21829-1-AP (GLUT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 21829-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21829-1-AP (GLUT1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21829-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21829-1-AP (GLUT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 21829-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21829-1-AP (GLUT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 21829-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GLUT1 antibody (21829-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 21829-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GLUT1 antibody (21829-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21829-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GLUT1 (21829-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: unboiled HT-29 cells, 37°C incubated mouse colon tissue
• Positive IHC detected in: rat brain tissue, human lung cancer tissue, human cervical cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

For optimal WB detection with 21829-1-AP, we recommend to avoid boiling the sample after lysis.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 227 publications below
• IHC: See 45 publications below
• IF: See 40 publications below
• FC: See 1 publications below
• ChIP: See 1 publications below

Product Information

21829-1-AP targets GLUT1 in WB, IHC, IF/ICC, IF-P, FC (Intra), ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, goat, lasiopodomys brandtii
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLUT1 fusion protein Ag16282
• Full Name: solute carrier family 2 (facilitated glucose transporter), member 1
• Calculated Molecular Weight: 492 aa, 54 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC121804
• Gene Symbol: GLUT1
• Gene ID (NCBI): 6513
• RRID: AB_10837075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucose transporter 1 (GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein responsible for the transport of glucose in many cell types and across the blood-brain barrier.

What is the molecular weight of GLUT1? Is GLUT1 post-translationally modified?

There are two forms of GLUT1 transporter that differ in their molecular weight. The 45-kDa form is found in glial cells, while the 55-kDa form is present in the endothelial cells regulating glucose transport over the blood-brain and blood-tissue barriers (PMID: 9630522). N-glycosylation of asparagine at position 42 is the only known post-translation modification of GLUT1 (PMID: 3839598).

What is the subcellular localization of GLUT1?

Glucose transporters, including GLUT1, are multiple-pass integral membrane proteins. GLUT1 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes.

What molecules can be transported by GLUT1?

The main substrate of GLUT1 transport is glucose, but it can also transport galactose, mannose, glucosamine, and reduced ascorbate.

What is the tissue expression pattern of GLUT1?

GLUT1 is expressed by many cell types but the highest levels are observed in erythrocytes and in the central nervous system (astrocytes). GLUT1 is responsible for glucose transfer across the blood-brain and blood-tissue barriers, including placental transport.

Publications

Species	Application	Title
human	IF	Adv Mater; Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration; Authors - Zhuo Li
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
mouse	WB,IHC	Int J Oral Sci; Transcriptional activation of glucose transporter 1 in orthodontic tooth movement-associated mechanical response.; Authors - Yu Wang
mouse	IF	ACS Nano; Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer; Authors - Lingtong Meng
mouse	IF	Neuron; Sympathetic nerve-enteroendocrine L cell communication modulates GLP-1 release, brain glucose utilization, and cognitive function; Authors - Wenran Ren
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-04-2024)

Nice Westernblots in NRVCMs

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCMs

FH

Hua (Verified Customer) (02-14-2023)

Good antibody working for WB with mouse liver samples. However, boiling mouse colon and brain tissues results in no band observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: mouse liver, brain, and colon

GLUT1 Antibody Western Blot validation (1:4000 dilution) in mouse liver, brain, and colon (Cat no:21829-1-AP)

FH

William (Verified Customer) (10-26-2021)

Very clear staining in IHC in rat brain tissue (Wistar) at a concentration of 1:100, very well localised to blood vessels. Not tested yet at different concentrations

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Rat brain

FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 1 µl/ million cells with Glut-1 antibody (21829-1-AP) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody alone and in red primary (21829-1-AP) +secondary antibody

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1µl/10^6cells
• Cell Tissue Type: B cells

GLUT1 Antibody Flow Cytometry validation (1µl/10^6cells dilution) in B cells (Cat no:21829-1-AP)

FH

Susan (Verified Customer) (11-19-2019)

20ug of HeLa cells overexpressing Glut1-GFP. Blocked with 5% milk in 0.1% TBST and incubated overnight at 4 degrees with rocking.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa

GLUT1 Antibody Western Blot,Immunofluorescence, validation (1:2000 dilution) in HeLa (Cat no:21829-1-AP)

FH

Kishor (Verified Customer) (12-13-2018)

I got good results in cultured cells but I could not see any results with rat liver protein.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

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