Anticorps Polyclonal de lapin anti-TOLLIP

• Phare
• Validé par KD/KO

TOLLIP Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 11315-1-AP

Synonymes

IL 1RAcPIP, toll interacting protein, TOLLIP

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Galerie de données de validation

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  WB analysis of PC-3 using 11315-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11315-1-AP

  WB result of TOLLIP antibody (11315-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOLLIP transfected HepG2 cells.

• 

  WB analysis using 11315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11315-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11315-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11315-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11315-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11315-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11315-1-AP (TOLLIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11315-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11315-1-AP (TOLLIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 11315-1-AP

  Immunofluorescent analysis of PC-3 cells using 11315-1-AP (TOLLIP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules PC-3, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique humain
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11315-1-AP cible TOLLIP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TOLLIP Protéine recombinante Ag1864
• Nom complet: toll interacting protein
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC012057
• Symbole du gène: TOLLIP
• Identification du gène (NCBI): 54472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

Publications

Species	Application	Title
human	WB	Autophagy; TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression; Authors - Tai-Mei Zhang
human	WB	PLoS Pathog; The MGF300-2R protein of African swine fever virus is associated with viral pathogenicity by promoting the autophagic degradation of IKKα and IKKβ through the recruitment of TOLLIP; Authors - Tao Wang
pig	WB	J Virol; African Swine Fever Virus L83L Negatively Regulates the cGAS-STING-Mediated IFN-I Pathway by Recruiting Tollip To Promote STING Autophagic Degradation; Authors - Mingyang Cheng
human	WB	mBio; The Globular C1q Receptor Is Required for Epidermal Growth Factor Receptor Signaling during Candida albicans Infection.; Authors - Quynh T Phan ; KD Validated
human	WB	Nat Commun; Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy; Authors - Shouheng Jin ; KD Validated
rat	IF	Gut Microbes; Lactiplantibacillus plantarum 299v supplementation modulates β-cell ER stress and antioxidative defense pathways and prevents type 1 diabetes in gluten-free BioBreeding rats; Authors - Pinar Sargin

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