IRAK4 Polyclonal antibody

IRAK4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 18221-1-AP

Synonyms

IPD1, IRAK 4, IRAK4, NY REN 64, REN64

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Validation Data Gallery

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  WB analysis of Jurkat using 18221-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18221-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18221-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 18221-1-AP

  IP result of anti-IRAK4 (IP:18221-1-AP, 4ug; Detection:18221-1-AP 1:300) with K-562 cells lysate 4000ug.

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  IHC staining of human lung cancer using 18221-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18221-1-AP (IRAK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18221-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18221-1-AP (IRAK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 18221-1-AP is IRAK4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRAK4 fusion protein Ag12982
• Full Name: interleukin-1 receptor-associated kinase 4
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC013316
• Gene Symbol: IRAK4
• Gene ID (NCBI): 51135
• RRID: AB_2878519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Integrated characterization of hepatobiliary tumor organoids provides a potential landscape of pharmacogenomic interactions; Authors - Yanjing Zhu
	WB	Mol Neurobiol; Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment; Authors - Liyun Deng
	WB	Front Pharmacol; Euphorbia Factor L2 ameliorates the Progression of K/BxN Serum-Induced Arthritis by Blocking TLR7 Mediated IRAK4/IKKβ/IRF5 and NF-kB Signaling Pathways.; Authors - Jing Tang
sheep	WB	Mediators Inflamm; Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.; Authors - Zhongjia Jiang
	WB	Evid Based Complement Alternat Med; Extracts of Salvia-Nelumbinis Naturalis Ameliorate Nonalcoholic Steatohepatitis via Inhibiting Gut-Derived Endotoxin Mediated TLR4/NF-κB Activation.; Authors - Xiangbing Shu
human	WB	Int Immunopharmacol; Herbal compound cepharanthine attenuates inflammatory arthritis by blocking macrophage M1 polarization; Authors - Chenyang Lu