Anticorps Polyclonal de lapin anti-TLR5

TLR5 Polyclonal Antibody for IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 19810-1-AP

Synonymes

SLEB1, TIL3, TLR5, toll like receptor 5

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Galerie de données de validation

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  IP experiment of HeLa using 19810-1-AP

  IP result of anti-TLR5 (IP:19810-1-AP, 4ug; Detection:19810-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human small intestine using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19810-1-AP (TLR5 antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human small intestine using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19810-1-AP (TLR5 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human small intestine using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19810-1-AP (TLR5 antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human tonsillitis using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19810-1-AP (TLR5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19810-1-AP (TLR5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19810-1-AP (TLR5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19810-1-AP (TLR5 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu d'amygdalite humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Informations sur le produit

19810-1-AP cible TLR5 dans les applications de WB, IP, IF, FC, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TLR5 Protéine recombinante Ag13840
• Nom complet: toll-like receptor 5
• Masse moléculaire calculée: 858 aa, 98 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC109119
• Symbole du gène: TLR5
• Identification du gène (NCBI): 7100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.

Publications

Species	Application	Title
mouse	WB	Theranostics; Prussian blue nanozyme-mediated nanoscavenger ameliorates acute pancreatitis via inhibiting TLRs/NF-κB signaling pathway.; Authors - Xue Xie
mouse	FC	Int J Mol Sci; Myeloid-Derived Suppressor Cells Gain Suppressive Function during Neonatal Bacterial Sepsis.; Authors - Jordan K Vance
rat	IHC, IF	Int J Mol Sci; Cross-Talk of Toll-Like Receptor 5 and Mu-Opioid Receptor Attenuates Chronic Constriction Injury-Induced Mechanical Hyperalgesia through a Protein Kinase C Alpha-Dependent Signaling.; Authors - Ching Chang
human	IHC	EBioMedicine; Roseburia intestinalis stimulates TLR5-dependent intestinal immunity against Crohn's disease; Authors - Zhaohua Shen
human	IHC	Oncotarget; Toll-like receptor 5 gene polymorphism is associated with breast cancer susceptibility.; Authors - Chen Shuang
human	WB	Cells; Fibronectin Functions as a Selective Agonist for Distinct Toll-like Receptors in Triple-Negative Breast Cancer.; Authors - Anthony Ambesi

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