TLR4 Monoclonal antibody
TLR4 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
3G9A4
Cat no : 66350-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | RAW 264.7 cells, pig liver tissue, NIH/3T3 cells, rat liver tissue |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 81 publications below |
IHC | See 4 publications below |
IF | See 9 publications below |
IP | See 1 publications below |
CoIP | See 2 publications below |
Product Information
66350-1-Ig targets TLR4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | TLR4 fusion protein Ag13857 |
Full Name | toll-like receptor 4 |
Calculated molecular weight | 839 aa, 96 kDa |
Observed molecular weight | 100-110 kDa |
GenBank accession number | BC117422 |
Gene symbol | TLR4 |
Gene ID (NCBI) | 7099 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.
Protocols
Product Specific Protocols | |
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WB protocol for TLR4 antibody 66350-1-Ig | Download protocol |
IHC protocol for TLR4 antibody 66350-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Biomater An injectable co-assembled hydrogel blocks reactive oxygen species and inflammation cycle resisting myocardial ischemia-reperfusion injury. | ||
Cell Rep METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling | ||
Int J Biol Macromol Angelica sinensis aboveground part polysaccharide and its metabolite 5-MT ameliorate colitis via modulating gut microbiota and TLR4/MyD88/NF-κB pathway | ||
Gut Microbes Microbial hydrogen economy alleviates colitis by reprogramming colonocyte metabolism and reinforcing intestinal barrier. | ||
J Control Release Inhalation of MSC-EVs is a noninvasive strategy for ameliorating acute lung injury. | ||
Front Bioeng Biotechnol Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts. |