TLR4 Monoclonal antibody

TLR4 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G9A4

Cat no : 66350-1-Ig

Synonyms

ARMD10, CD284, hToll, TLR4, TOLL, toll like receptor 4



Tested Applications

Positive WB detected inRAW 264.7 cells, pig liver tissue, NIH/3T3 cells, rat liver tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66350-1-Ig targets TLR4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TLR4 fusion protein Ag13857
Full Name toll-like receptor 4
Calculated molecular weight 839 aa, 96 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC117422
Gene symbol TLR4
Gene ID (NCBI) 7099
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

Protocols

Product Specific Protocols
WB protocol for TLR4 antibody 66350-1-IgDownload protocol
IHC protocol for TLR4 antibody 66350-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomater Adv

Monosodium urate crystals with controlled shape and aspect ratio for elucidating the pathological progress of acute gout.

Authors - Chen Chen
ratWB

Acta Biomater

An injectable co-assembled hydrogel blocks reactive oxygen species and inflammation cycle resisting myocardial ischemia-reperfusion injury.

Authors - Xu Liao
humanWB

Front Bioeng Biotechnol

Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.

Authors - Kaile Zhang
ratWB

Exp Biol Med (Maywood)

Oxymatrine suppresses IL-1β-induced degradation of the nucleus pulposus cell and extracellular matrix through the TLR4/NF-κB signaling pathway.

Authors - Kang Wei
humanWB

Stem Cells Int

EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4/MyD88/NF-κB Pathway.

Authors - Pengcheng Wang
ratWB

Pak J Pharm Sci

Platycodon grandiflorum extract attenuates lipopolysaccharide-induced acute lung injury via TLR4/NF-κBp65 pathway in rats.

Authors - Li Si-Cong