TLR4 Monoclonal antibody

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  WB analysis of RAW 264.7 using 66350-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 66350-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66350-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66350-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66350-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66350-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

TLR4 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66350-1-Ig

Clone No.3G9A4

Publications(187)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig and More (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

toll like receptor 4, TOLL, hToll, CD284, ARMD10

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, pig liver tissue, NIH/3T3 cells, rat liver tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 172 publications below
• IHC: See 13 publications below
• IF: See 19 publications below
• IP: See 2 publications below
• CoIP: See 5 publications below

Product Information

66350-1-Ig targets TLR4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, pig, rabbit, zebrafish, sheep
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TLR4 fusion protein Ag13857
• Full Name: toll-like receptor 4
• Calculated Molecular Weight: 839 aa, 96 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC117422
• Gene Symbol: TLR4
• Gene ID (NCBI): 7099
• RRID: AB_2881730
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00206
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLR4, also named CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
human	WB	Adv Sci (Weinh); OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression; Authors - Xingran Li
rat	WB	Acta Biomater; An injectable co-assembled hydrogel blocks reactive oxygen species and inflammation cycle resisting myocardial ischemia-reperfusion injury.; Authors - Xu Liao
rat	WB	Acta Biomater; Mechanically tough, adhesive, self-healing hydrogel promotes annulus fibrosus repair via autologous cell recruitment and microenvironment regulation; Authors - Zhenyuan Wei
mouse	WB	Cell Rep; METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling; Authors - Shuhua Luo
pig	WB	Int J Biol Macromol; Angelica sinensis aboveground part polysaccharide and its metabolite 5-MT ameliorate colitis via modulating gut microbiota and TLR4/MyD88/NF-κB pathway; Authors - Yuan-Feng Zou