TLR7 Polyclonal antibody

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  WB analysis of mouse lung using 17232-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17232-1-AP (TLR7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17232-1-AP

  WB result of TLR7 antibody (17232-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLR7 transfected Jurkat cells.

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  IHC staining of human lung using 17232-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17232-1-AP (TLR7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 17232-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17232-1-AP (TLR7 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of RAW 264.7 using 17232-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TLR7 antibody (17232-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

TLR7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17232-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UNQ248/PRO285, Toll-like receptor 7, toll like receptor 7

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Tested Applications

• Positive WB detected in: mouse lung tissue, Jurkat cells
• Positive IHC detected in: human lung tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

17232-1-AP targets TLR7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TLR7 fusion protein Ag11073
• Full Name: toll-like receptor 7
• Calculated Molecular Weight: 1049 aa, 121 kDa
• Observed Molecular Weight: 121 kDa, 100 kDa
• GenBank Accession Number: BC033651
• Gene Symbol: TLR7
• Gene ID (NCBI): 51284
• RRID: AB_2203464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYK1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Among the TLR family, TLR7 (toll-like receptor 7) is an endosomal innate immune sensor that can detect single-stranded ribonucleic acid. Constitutive expression of TLR7 is pre-dominant in dendritic cells and B cells compared to other immune cells. Low levels of TLR7 expression have been observed in non-immune cells, such as hepatocytes, epithelial cells, and keratinocytes (PMID: 37596656).

Publications

Species	Application	Title
human	IHC	Nat Commun; TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase; Authors - Gang Liu
rat	WB	Acta Physiol (Oxf); Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.; Authors - Li Zhang
human	WB	Stem Cell Res Ther; Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.; Authors - Ruijie Zhang ; KD Validated
mouse	WB,IF	Mol Neurobiol; Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment; Authors - Liyun Deng
mouse	WB	Front Immunol; Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations.; Authors - Lujie Yang
rat	WB	Mol Cell Endocrinol; Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.; Authors - Dongmin Li