TLR7 Polyclonal antibody

• Featured Product
• KD/KO Validated

TLR7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 17232-1-AP

Synonyms

TLR7, toll like receptor 7

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Validation Data Gallery

• 

  WB analysis of mouse lung using 17232-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17232-1-AP (TLR7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17232-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17232-1-AP (TLR7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 17232-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17232-1-AP (TLR7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 17232-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17232-1-AP (TLR7 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue, Jurkat cells
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below

Product Information

17232-1-AP targets TLR7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TLR7 fusion protein Ag11073
• Full Name: toll-like receptor 7
• Calculated molecular weight: 1049 aa, 121 kDa
• Observed molecular weight: 121 kDa
• GenBank accession number: BC033651
• Gene symbol: TLR7
• Gene ID (NCBI): 51284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Acta Physiol (Oxf); Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.; Authors - Li Zhang
human	WB	Stem Cell Res Ther; Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.; Authors - Ruijie Zhang ; KD Validated
mouse	WB	Front Immunol; Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations.; Authors - Lujie Yang
rat	WB	Mol Cell Endocrinol; Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.; Authors - Dongmin Li
mouse	WB	Evid Based Complement Alternat Med; Lignans from Mosla scabra Ameliorated Influenza A Virus-Induced Pneumonia via Inhibiting Macrophage Activation; Authors - Wei Cai
human	WB	Exp Ther Med; Effect of HMGB1 on monocyte immune function in respiratory syncytial virus bronchiolitis.; Authors - Mingyang Niu

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