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  • KD/KO Validated

TLR7 Polyclonal antibody

TLR7 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 17232-1-AP


TLR7, toll like receptor 7

Tested Applications

Positive WB detected inmouse lung tissue, Jurkat cells
Positive IHC detected inhuman lung tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17232-1-AP targets TLR7 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLR7 fusion protein Ag11073
Full Name toll-like receptor 7
Calculated Molecular Weight 1049 aa, 121 kDa
Observed Molecular Weight 121 kDa, 100 kDa
GenBank Accession NumberBC033651
Gene Symbol TLR7
Gene ID (NCBI) 51284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Among the TLR family, TLR7 (toll-like receptor 7) is an endosomal innate immune sensor that can detect single-stranded ribonucleic acid. Constitutive expression of TLR7 is pre-dominant in dendritic cells and B cells compared to other immune cells. Low levels of TLR7 expression have been observed in non-immune cells, such as hepatocytes, epithelial cells, and keratinocytes (PMID: 37596656).


Product Specific Protocols
WB protocol for TLR7 antibody 17232-1-APDownload protocol
IHC protocol for TLR7 antibody 17232-1-APDownload protocol
IF protocol for TLR7 antibody 17232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase

Authors - Gang Liu

Acta Physiol (Oxf)

Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.

Authors - Li Zhang

Stem Cell Res Ther

Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.

Authors - Ruijie Zhang
  • KD Validated

Mol Neurobiol

Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment

Authors - Liyun Deng

Front Immunol

Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations.

Authors - Lujie Yang

Mol Cell Endocrinol

Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.

Authors - Dongmin Li