TLR3 Polyclonal antibody

TLR3 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, FC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 17766-1-AP

Synonyms

CD283, TLR3, toll like receptor 3

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Validation Data Gallery

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  IHC staining of human tonsillitis using 17766-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17766-1-AP (TLR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17766-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17766-1-AP (TLR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 17766-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TLR3 antibody (17766-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 5 publications below

Product Information

The immunogen of 17766-1-AP is TLR3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TLR3 fusion protein Ag12171
• Full Name: toll-like receptor 3
• Calculated molecular weight: 904 aa, 104 kDa
• GenBank accession number: BC094737
• Gene symbol: TLR3
• Gene ID (NCBI): 7098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLR3 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production and dendritic cell (DC) maturation (PMID: 18262679).

Publications

Species	Application	Title
human,mouse	WB,IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
human	WB	J Cell Mol Med; Down-regulation of miR-10a-5p in synoviocytes contributes to TBX5-controlled joint inflammation.; Authors - Nazim Hussain
mouse	WB	Phytomedicine; Wu-Mei-Wan prevents high-fat diet-induced obesity by reducing white adipose tissue and enhancing brown adipose tissue function.; Authors - Fan Wu
human	WB	Front Cell Infect Microbiol; Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.; Authors - Matthew Charman
human	WB	RNA; MiR-146a down-regulates inflammatory response by targeting TLR3 and TRAF6 in Coxsackievirus B infection.; Authors - Yanru Fei
mouse	WB	J Appl Toxicol; Intratracheal instillation of graphene oxide decreases anti-virus responses and lipid contents via suppressing Toll-like receptor 3 in mouse livers.; Authors - Xiaomin Tang

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