TLR3 Polyclonal antibody

TLR3 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, FC

Conjugate

Unconjugated

Cat no : 17766-1-AP

Synonyms

CD283, TLR3, toll like receptor 3



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17766-1-AP is TLR3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLR3 fusion protein Ag12171
Full Name toll-like receptor 3
Calculated molecular weight 904 aa, 104 kDa
GenBank accession numberBC094737
Gene symbol TLR3
Gene ID (NCBI) 7098
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLR3 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production and dendritic cell (DC) maturation (PMID: 18262679).

Protocols

Product Specific Protocols
IHC protocol for TLR3 antibody 17766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncotarget

Comparative study of macrophages in naked mole rats and ICR mice.

Authors - Jishuai Cheng
humanIHC

Viral Immunol

A Comparison of Splenic Pathologic Change and Immune Function in HBV-Related Portal Hypertension and Chinese Budd-Chiari Syndrome Patients with Hypersplenism.

Authors - Suxin Li
humanWB

Toxicol Res (Camb)

Transcriptomic-based toxicological investigations of graphene oxide with modest cytotoxicity to human umbilical vein endothelial cells: changes of Toll-like receptor signaling pathways.

Authors - Yingmei Luo
humanWB

RNA

MiR-146a down-regulates inflammatory response by targeting TLR3 and TRAF6 in Coxsackievirus B infection.

Authors - Yanru Fei
humanWB

Front Cell Infect Microbiol

Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.

Authors - Matthew Charman
mouseWB

Phytomedicine

Wu-Mei-Wan prevents high-fat diet-induced obesity by reducing white adipose tissue and enhancing brown adipose tissue function.

Authors - Fan Wu