TLR6 Polyclonal antibody

• Featured Product
• KD/KO Validated

TLR6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 22240-1-AP

Synonyms

Toll-like receptor 6, toll like receptor 6, CD286

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Validation Data Gallery

• 

  WB analysis of RAW 264.7 using 22240-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22240-1-AP (TLR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 22240-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22240-1-AP (TLR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 22240-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22240-1-AP (TLR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22240-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22240-1-AP (TLR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 22240-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 22240-1-AP (TLR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, human brain tissue, mouse thymus tissue, Jurkat cells, HT-1080 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below

Product Information

22240-1-AP targets TLR6 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TLR6 fusion protein Ag17686
• Full Name: toll-like receptor 6
• Calculated Molecular Weight: 480 aa, 56 kDa
• Observed Molecular Weight: 95-110 kDa
• GenBank Accession Number: BC111755
• Gene Symbol: TLR6
• Gene ID (NCBI): 10333
• RRID: AB_11232032
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLR6, also known as CD286, belongs to the Toll-like receptor (TLR) family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR6 can form a heterodimer with TLR2. TLR6 and TLR2 both are recruited to the macrophage phagosome, where they recognize peptidoglycan, a Gram-positive pathogen component (PMID: 11095740). TLR6 interacts with CD36, following CD36 stimulation by oxLDL or amyloid-beta 42, and forms a heterodimer with TLR4. The trimeric complex is internalized and triggers an inflammatory response.

Publications

Species	Application	Title
mouse	WB	ACS Nano; Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment.; Authors - Han Lin
human,mouse	WB	BMC Med; HBeAg mediates inflammatory functions of macrophages by TLR2 contributing to hepatic fibrosis.; Authors - Xiaoyu Xie ; KD Validated
human	WB	J Med Chem; Design, Synthesis, and Structure-Activity Relationship of N-Aryl-N'-(thiophen-2-yl)thiourea Derivatives as Novel and Specific Human TLR1/2 Agonists for Potential Cancer Immunotherapy.; Authors - Zhipeng Chen
human		Bioorg Chem; Synthesis, evaluation and molecular dynamics study of human toll-like receptor 2/6 specific monoacyl lipopeptides as candidate immunostimulants; Authors - Yueyue Zhu
rat	WB	J Gerontol A Biol Sci Med Sci; METTL3 promotes nucleus pulposus cell senescence in intervertebral disc degeneration by regulating TLR2 m6A methylation and gut microbiota; Authors - Shuangfei Ni
human	WB	Eur J Med Chem; Structure-activity relationship study and biological evaluation of SAC-Garlic acid conjugates as novel anti-inflammatory agents.; Authors - Jingjie Bi

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