Anticorps Polyclonal de lapin anti-TLE3

• Phare
• Validé par KD/KO

TLE3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (1)

N° de cat : 11372-1-AP

Synonymes

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

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Galerie de données de validation

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  WB analysis using 11372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11372-1-AP

  WB result of TLE3 antibody (11372-1-AP, 1:500) with si-Control and si-TLE3 transfected HEK293 cells.

• 

  IP experiment of HepG2 using 11372-1-AP

  IP result of anti-TLE3 (IP:11372-1-AP, 4ug; Detection:11372-1-AP 1:2000) with HepG2 cells lysate 1080 ug.

• 

  IHC staining of human breast cancer using 11372-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11372-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11372-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11372-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11372-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (11372-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• CoIP: See 2 publications below
• ChIP: See 7 publications below

Informations sur le produit

11372-1-AP cible TLE3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TLE3 Protéine recombinante Ag1930
• Nom complet: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Masse moléculaire calculée: 772 aa, 83 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC015729
• Symbole du gène: TLE3
• Identification du gène (NCBI): 7090
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane

Publications

Species	Application	Title
human	WB,CoIP,ChIP	Nature; Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer.; Authors - Abhijit Parolia ; KD Validated
mouse	WB	Cell Stem Cell; Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription.; Authors - Qi Li
mouse	WB,ChIP	Cell Metab; TLE3 is a dual-function transcriptional coregulator of adipogenesis.; Authors - Villanueva Claudio J CJ
mouse	WB	Cell Metab; Adipose subtype-selective recruitment of TLE3 or Prdm16 by PPARγ specifies lipid storage versus thermogenic gene programs.; Authors - Villanueva Claudio J CJ ; KO Validated
human	WB,chIP	J Clin Invest; Small molecule JQ1 promotes prostate cancer invasion via BET-independent inactivation of FOXA1.; Authors - Leiming Wang
mouse	chIP	J Exp Med; Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity.; Authors - Shaojun Xing ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tongbin (Verified Customer) (08-05-2020)

I did western blots using mouse heart samples. I observed a TLE3 band with correct molecular weight of about 80kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

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