HESX1 Polyclonal antibody

HESX1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17927-1-AP

Synonyms

ANF, hAnf, HESX homeobox 1, HESX1, Homeobox protein ANF, RPX

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Validation Data Gallery

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  IHC staining of mouse testis using 17927-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17927-1-AP (HESX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17927-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17927-1-AP (HESX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17927-1-AP targets HESX1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HESX1 fusion protein Ag12348
• Full Name: HESX homeobox 1
• Calculated Molecular Weight: 185 aa, 21 kDa
• GenBank Accession Number: BC093979
• Gene Symbol: HESX1
• Gene ID (NCBI): 8820
• RRID: AB_2118120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HESX1 (Homeobox gene expressed in stem cells 1) is a homeobox transcriptional repressor that was initially isolated from murine embryonic stem cells (ESCs). Hesx1 is crucial for proper forebrain and pituitary development in both mice and humans, and inactivating mutations in HESX1 underlie septo-optic dysplasia.