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Anticorps Monoclonal anti-TGN46

TGN46 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F6D5

N° de cat : 66477-1-Ig

Synonymes

Golgi marker, TGN38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

Galerie de données de validation

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  • Western Blot (WB) analysis of HeLa cells using TGN46 Monoclonal antibody (66477-1-Ig)

    WB analysis of HeLa using 66477-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TGN46 Monoclonal antibody (66477-1-Ig)

    WB analysis using 66477-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human breast cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human breast cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human liver cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human liver cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Applications testées

Résultats positifs en WBcellules A549, cellules HeLa, cellules HepG2, cellules L02, cellules LNCaP, cellules SMMC-7721, tissu hépatique de porc
Résultats positifs en IHCtissu de cancer du sein humain, tissu de cancer du foie humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochimie (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66477-1-Ig cible TGN46 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène TGN46 Protéine recombinante Ag4470
Nom complet trans-golgi network protein 2
Masse moléculaire calculée 447 aa, 47 kDa
Poids moléculaire observé 90 kDa
Numéro d’acquisition GenBankBC028219
Symbole du gène TGOLN2
Identification du gène (NCBI) 10618
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Protocole

Product Specific Protocols
WB protocol for TGN46 antibody 66477-1-IgDownload protocol
IHC protocol for TGN46 antibody 66477-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Karla (Verified Customer) (12-20-2022)

Proteintech has the best for IMF. Made my images clear and was picked up easily on the microscope.

  • Applications: Immunofluorescence