AP1M1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AP1M1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 12112-1-AP

Synonyms

AP 1 complex subunit mu 1, AP mu chain family member mu1A, AP1M1, AP47, CLAPM2, CLTNM, MU 1A, Mu adaptin 1, Mu1A adaptin

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Validation Data Gallery

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  WB analysis of Hacat cells using 12112-1-AP

  WB resut of AP1M1 antibody (12112-1-AP, 1:500) with siRNA cell lysate.

• 

  WB analysis of human brain using 12112-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 12112-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12112-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 12112-1-AP

  IP result of anti-AP1M1 (IP:12112-1-AP, 4ug; Detection:12112-1-AP 1:500) with SH-SY5Y cells lysate 2500ug.

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Tested Applications

• Positive WB detected in: human brain tissue, Hacat cells
• Positive IP detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

12112-1-AP targets AP1M1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP1M1 fusion protein Ag2757
• Full Name: adaptor-related protein complex 1, mu 1 subunit
• Calculated Molecular Weight: 423 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC017469
• Gene Symbol: AP1M1
• Gene ID (NCBI): 8907
• RRID: AB_2058339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Plasma membrane contributes to the formation of pre-autophagosomal structures.; Authors - Ravikumar Brinda B ; KD Validated
human	IF,CoIP	Int J Mol Sci; Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer; Authors - Hsien-Neng Huang
human	WB,IF	Traffic; AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins.; Authors - Mathilde Bonnemaison ; KD Validated
	WB	J Biol Chem; Adaptor Protein-1 Complex Affects the Endocytic Trafficking and Function of Peptidylglycine α-Amidating Monooxygenase, a Luminal Cuproenzyme.; Authors - Mathilde L Bonnemaison ; KD Validated
human,mouse	WB	Biomed Pharmacother; Dioscin alleviates Alzheimer's disease through regulating RAGE/NOX4 mediated oxidative stress and inflammation.; Authors - Linshu Guan
canine, mouse	WB	PLoS One; Basolateral BMP signaling in polarized epithelial cells.; Authors - Saitoh Masao M ; KD Validated

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