GORASP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GORASP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

68

Reviews (4)

Cat no : 10598-1-AP

Synonyms

GRS2, GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2

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Validation Data Gallery

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  WB analysis using 10598-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10598-1-AP

  WB result of GORASP2 antibody (10598-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GORASP2 transfected HeLa cells.

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  WB analysis using 10598-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10598-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10598-1-AP

  IP result of anti-GORASP2 (IP:10598-1-AP, 3ug; Detection:10598-1-AP 1:700) with HeLa cells lysate 1320ug.

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  IHC staining of human colon cancer using 10598-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10598-1-AP (GORASP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 10598-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10598-1-AP (GORASP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10598-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10598-1-AP (GORASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10598-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (10598-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL555-Phalloidin (orange).

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Tested Applications

• Positive WB detected in: COLO 320 cells, rat testis tissue, HeLa cells, Raji cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 26 publications below
• WB: See 49 publications below
• IHC: See 2 publications below
• IF: See 33 publications below

Product Information

10598-1-AP targets GORASP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GORASP2 fusion protein Ag0950
• Full Name: golgi reassembly stacking protein 2, 55kDa
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC007770
• Gene Symbol: GORASP2
• Gene ID (NCBI): 26003
• RRID: AB_2113473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Publications

Species	Application	Title
human	IHC	Nature; Cell stress in cortical organoids impairs molecular subtype specification.; Authors - Aparna Bhaduri
human	WB,IF	Mol Cell; An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.; Authors - Julian Nüchel
human	WB	Dev Cell; A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells.; Authors - Francesco Ferraro ; KD Validated
		Nat Commun; Land-locked mammalian Golgi reveals cargo transport between stable cisternae.; Authors - Myun Hwa Dunlop
mouse	IF	J Clin Invest; Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy; Authors - Frank L. van de Veerdonk ; KD Validated
human	WB,IF	J Cell Biol; GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking.; Authors - Xiang Yi Y ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cassan (Verified Customer) (06-16-2022)

Verified good working antibody

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: brain

GORASP2 Antibody Immunofluorescence validation (1 to 200 dilution) in brain (Cat no:10598-1-AP)

FH

Hai (Verified Customer) (07-09-2021)

I used 1:400 dilution in 2%BSA/PBS and got strong signals. The cells in the image I uploaded was stained with mouse-Tubulin- AF633 and rabbit-GORASP2- AF488 and DAPI.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Melanoma cells

GORASP2 Antibody Immunofluorescence validation (1:400 dilution) in Melanoma cells (Cat no:10598-1-AP)

FH

Ana (Verified Customer) (03-14-2021)

GORASP, golgi labeling in human primary fibroblasts. PFA 4% fixed cells. 1:100 primary ab in 5% GS PBST (0.1% triton) O/N 4ºC incubation. 1:500 secondary ab Alexafluor 488 1h RT.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Primary Fibroblasts

GORASP2 Antibody Immunofluorescence validation (1:100 dilution) in Human Primary Fibroblasts (Cat no:10598-1-AP)

FH

Uxoa (Verified Customer) (02-18-2020)

GORASP2 (alexa fluor 488, green), Vimentin (alexa fluor 647, white), DAPI (blue). Primary fibroblasts fixed with 4% PFA 15min. 1:100 GORASP2 ab dilution, in PBST (0.1% triton) + 10% DS O/N 4ºC.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Primary human fibroblasts

GORASP2 Antibody Immunofluorescence, validation (1:100 dilution) in Primary human fibroblasts (Cat no:10598-1-AP)

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