GORASP2 Polyclonal antibody

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• GORASP2 Polyclonal antibody (10598-1-AP)

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GORASP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10598-1-AP

Publications(88)

Reviews (5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Golgi phosphoprotein 6, Golgi reassembly-stacking protein 2, Golgi reassembly-stacking protein of 55 kDa, GOLPH6, GRASP55

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  WB analysis using 10598-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HeLa using 10598-1-AP

  WB result of GORASP2 antibody (10598-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GORASP2 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat testis using 10598-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10598-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 10598-1-AP

  IP result of anti-GORASP2 (IP:10598-1-AP, 3ug; Detection:10598-1-AP 1:700) with HeLa cells lysate 1320ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human colon cancer using 10598-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10598-1-AP (GORASP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 10598-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10598-1-AP (GORASP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 10598-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10598-1-AP (GORASP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 10598-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (10598-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-Phalloidin (orange).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 10598-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (10598-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 10598-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10598-1-AP (GORASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, rat testis tissue, Raji cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10598-1-AP targets GORASP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0950

  Product name: Recombinant human GORASP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 152-452 aa of BC007770

  Sequence: LIETHEAKPLKLYVYNTDTDNCREVIITPNSAWGGEGSLGCGIGYGYLHRIPTRPFEEGKKISLPGQMAGTPITPLKDGFTEVQLSSVNPPSLSPPGTTGIEQSLTGLSISSTPPAVSSVLSTGVPTVPLLPPQVNQSLTSVPPMNPATTLPGLMPLPAGLPNLPNLNLNLPAPHIMPGVGLPELVNPGLPPLPSMPPRNLPGIAPLPLPSEFLPSFPLVPESSSAASSGELLSSLPPTSNAPSDPATTTAKADAASSLTVDVTPPTAKAPTTVEDRVGDSTPVSEKPVSAAVDANASESP

• Full Name: golgi reassembly stacking protein 2, 55kDa
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC007770
• Gene Symbol: GORASP2
• Gene ID (NCBI): 26003
• RRID: AB_2113473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H8Y8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Nature; Cell stress in cortical organoids impairs molecular subtype specification.; Authors - Aparna Bhaduri
human		Cell; Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging; Authors - Florian Schueder
human	IF	Cancer Res; Subcellular Redistribution of Endomembrane GPR15 Promotes NAD+-Mediated Metabolic Reprogramming and Boosts 5-FU Chemosensitivity in Colorectal Cancer.; Authors - Zhiying Yue
human	WB,IF	Autophagy; GORASP2/GRASP55 collaborates with the PtdIns3K UVRAG complex to facilitate autophagosome-lysosome fusion.; Authors - Xiaoyan Zhang ; KD Validated
human	WB	Autophagy; GORASP2 promotes phagophore closure and autophagosome maturation into autolysosomes; Authors - Yusheng Xing ; KD Validated
human	WB	Autophagy; TGFB1 is secreted through an unconventional pathway dependent on the autophagic machinery and cytoskeletal regulators.; Authors - Julian Nüchel ; KD Validated

Reviews

5.0 out of 5

5 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Laura (Verified Customer) (07-24-2025)

Great antibody! Worked well in IF and WB at recommended dilutions. Used for mouse cell lines.

• Applications: Western Blot, Immunofluorescence

Cassan (Verified Customer) (06-16-2022)

Verified good working antibody

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: brain

GORASP2 Antibody Immunofluorescence validation (1 to 200 dilution) in brain (Cat no:10598-1-AP)

Hai (Verified Customer) (07-09-2021)

I used 1:400 dilution in 2%BSA/PBS and got strong signals. The cells in the image I uploaded was stained with mouse-Tubulin- AF633 and rabbit-GORASP2- AF488 and DAPI.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Melanoma cells

GORASP2 Antibody Immunofluorescence validation (1:400 dilution) in Melanoma cells (Cat no:10598-1-AP)

Ana (Verified Customer) (03-14-2021)

GORASP, golgi labeling in human primary fibroblasts. PFA 4% fixed cells. 1:100 primary ab in 5% GS PBST (0.1% triton) O/N 4ºC incubation. 1:500 secondary ab Alexafluor 488 1h RT.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Primary Fibroblasts

GORASP2 Antibody Immunofluorescence validation (1:100 dilution) in Human Primary Fibroblasts (Cat no:10598-1-AP)

Uxoa (Verified Customer) (02-18-2020)

GORASP2 (alexa fluor 488, green), Vimentin (alexa fluor 647, white), DAPI (blue). Primary fibroblasts fixed with 4% PFA 15min. 1:100 GORASP2 ab dilution, in PBST (0.1% triton) + 10% DS O/N 4ºC.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Primary human fibroblasts

GORASP2 Antibody Immunofluorescence, validation (1:100 dilution) in Primary human fibroblasts (Cat no:10598-1-AP)