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  • KD/KO Validated

GORASP2 Polyclonal antibody

GORASP2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 10598-1-AP


Golgi phosphoprotein 6, GOLPH6, GORASP2, GRASP55, GRS2, p59

Tested Applications

Positive WB detected inCOLO 320 cells, rat testis tissue, HeLa cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10598-1-AP targets GORASP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GORASP2 fusion protein Ag0950
Full Name golgi reassembly stacking protein 2, 55kDa
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC007770
Gene Symbol GORASP2
Gene ID (NCBI) 26003
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.


Product Specific Protocols
WB protocol for GORASP2 antibody 10598-1-APDownload protocol
IHC protocol for GORASP2 antibody 10598-1-APDownload protocol
IF protocol for GORASP2 antibody 10598-1-APDownload protocol
IP protocol for GORASP2 antibody 10598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Cell stress in cortical organoids impairs molecular subtype specification.

Authors - Aparna Bhaduri

Mol Cell

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

Authors - Julian Nüchel

Dev Cell

A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells.

Authors - Francesco Ferraro
  • KD Validated

Nat Commun

Land-locked mammalian Golgi reveals cargo transport between stable cisternae.

Authors - Myun Hwa Dunlop

J Clin Invest

Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy

Authors - Frank L. van de Veerdonk
  • KD Validated

J Cell Biol

GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking.

Authors - Xiang Yi Y
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Cassan (Verified Customer) (06-16-2022)

Verified good working antibody

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1 to 200
  • Cell Tissue Type: brain
GORASP2 Antibody Immunofluorescence validation (1 to 200 dilution) in brain (Cat no:10598-1-AP)

Hai (Verified Customer) (07-09-2021)

I used 1:400 dilution in 2%BSA/PBS and got strong signals. The cells in the image I uploaded was stained with mouse-Tubulin- AF633 and rabbit-GORASP2- AF488 and DAPI.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Melanoma cells
GORASP2 Antibody Immunofluorescence validation (1:400 dilution) in Melanoma cells (Cat no:10598-1-AP)

Ana (Verified Customer) (03-14-2021)

GORASP, golgi labeling in human primary fibroblasts. PFA 4% fixed cells. 1:100 primary ab in 5% GS PBST (0.1% triton) O/N 4ºC incubation. 1:500 secondary ab Alexafluor 488 1h RT.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Primary Fibroblasts
GORASP2 Antibody Immunofluorescence validation (1:100 dilution) in Human Primary Fibroblasts (Cat no:10598-1-AP)

Uxoa (Verified Customer) (02-18-2020)

GORASP2 (alexa fluor 488, green), Vimentin (alexa fluor 647, white), DAPI (blue). Primary fibroblasts fixed with 4% PFA 15min. 1:100 GORASP2 ab dilution, in PBST (0.1% triton) + 10% DS O/N 4ºC.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Primary human fibroblasts
GORASP2 Antibody Immunofluorescence, validation (1:100 dilution) in Primary human fibroblasts (Cat no:10598-1-AP)