AP1M2 Polyclonal antibody

• Featured Product
• KD/KO Validated

AP1M2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10618-1-AP

Synonyms

AP1 mu2, AP-1 complex subunit mu-2, AP mu chain family member mu1B, AP 1 complex subunit mu 2, Adaptor-related protein complex 1 subunit mu-2

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Validation Data Gallery

• 

  WB analysis of mouse colon using 10618-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Hacat cells using 10618-1-AP

  WB result of AP1M2 antibody (10618-1-AP;1:500) with siRNA cell lysate.

• 

  WB analysis of BxPC-3 using 10618-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10618-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10618-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 10618-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 10618-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10618-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10618-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse colon tissue, Hacat cells
• Positive IHC detected in: human pancreas cancer tissue, human kidney tissue, human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10618-1-AP targets AP1M2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP1M2 fusion protein Ag0961
• Full Name: adaptor-related protein complex 1, mu 2 subunit
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC005021
• Gene Symbol: AP1M2
• Gene ID (NCBI): 10053
• RRID: AB_2242895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP1M2 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

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