Anticorps Polyclonal de lapin anti-CD71

• Phare
• Validé par KD/KO

CD71 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (4)

Applications

WB, IHC, IF/ICC, IP, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications

92

Avis (2)

N° de cat : 10084-2-AP

Synonymes

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

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Galerie de données de validation

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  WB analysis using 10084-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10084-2-AP (CD71 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10084-2-AP

  IP result of anti-CD71 (IP:10084-2-AP, 4ug; Detection:10084-2-AP 1:500) with HeLa cells lysate 2400ug.

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  IHC staining of human placenta using 10084-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10084-2-AP (CD71 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10084-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10084-2-AP (CD71 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10084-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CD71 antibody (10084-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules HeLa, cellules Jurkat, cellules K-562, tissu placentaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 75 publications below
• IHC: See 19 publications below
• IF: See 7 publications below

Informations sur le produit

10084-2-AP cible CD71 dans les applications de WB, IHC, IF/ICC, IP, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, poulet, singe, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD71 Protéine recombinante Ag0123
• Nom complet: transferrin receptor (p90, CD71)
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC001188
• Symbole du gène: CD71
• Identification du gène (NCBI): 7037
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publications

Species	Application	Title
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
human	IHC	Acta Neuropathol; Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.; Authors - Till S Zimmer
	WB,IHC	Cell Death Differ; Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis; Authors - Qing-Xin Ji
	WB,IF	J Nanobiotechnology; Glioma-targeted oxaliplatin/ferritin clathrate reversing the immunosuppressive microenvironment through hijacking Fe2+ and boosting Fenton reaction; Authors - Xue Li
	WB	Acta Biomater; Cyclosporine A-loaded apoferritin alleviates myocardial ischemia-reperfusion injury by simultaneously blocking ferroptosis and apoptosis of cardiomyocytes; Authors - Wenqiang Qian
human	IHC	Cancer Res; Intratumoral payload release influences the potency and bystander-killing effects of antibody-drug conjugates in preclinical models.; Authors - Fu Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nu (Verified Customer) (10-24-2023)

Although there is a band close to 100 kd, there are other even more stronger non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: postmortem human frontal cortex

CD71 Antibody Western Blot validation (1:1000 dilution) in postmortem human frontal cortex (Cat no:10084-2-AP)

FH

Sara (Verified Customer) (10-25-2020)

Really good signal in WB, it worked great, even though I loaded 1-2 µg of total cell lysate in some lanes. Not that good for IF, the signal was a bit fuzzy and all over the cell.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 WB / 1:100 IF
• Cell Tissue Type: B cells from mouse spleen

CD71 Antibody Western Blot,Immunofluorescence validation (1:1000 WB / 1:100 IF dilution) in B cells from mouse spleen (Cat no:10084-2-AP)

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