IREB2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IREB2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 23829-1-AP

Synonyms

ACO3, IRE BP 2, IREB2, Iron regulatory protein 2, IRP2, IRP2AD

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Validation Data Gallery

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  WB analysis of mouse lung using 23829-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 23829-1-AP (IREB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 23829-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23829-1-AP (IREB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 23829-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 23829-1-AP (IREB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 23829-1-AP

  Immunofluorescent analysis of A549 cells using 23829-1-AP (IREB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse lung tissue, A549 cells, PC-13 cells
• Positive IHC detected in: human spleen tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

23829-1-AP targets IREB2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IREB2 fusion protein Ag20653
• Full Name: iron-responsive element binding protein 2
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 90-105 kDa
• GenBank Accession Number: BC017880
• Gene Symbol: IREB2
• Gene ID (NCBI): 3658
• RRID: AB_2879331
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.

Publications

Species	Application	Title
mouse	WB	EMBO Rep; The deubiquitinase OTUD1 enhances iron transport and potentiates host antitumor immunity.; Authors - Jia Song
human	WB	Cell Mol Life Sci; Phenazine derivatives attenuate the stemness of breast cancer cells through triggering ferroptosis.; Authors - Yue Yang
human	WB,IF,CoIP	Free Radic Biol Med; Iron regulatory protein 2 is required for artemether -mediated anti-hepatic fibrosis through ferroptosis pathway.; Authors - Yujia Li ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis.; Authors - Haipeng Cheng
human	WB	Nutrients; Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells.; Authors - Xiaonan Yu
mouse	WB,IF	Neurochem Int; Activation of vitamin D receptor inhibits Tau phosphorylation is associated with reduction of iron accumulation in APP/PS1 transgenic mice.; Authors - Ting-Yao Wu