HFE Polyclonal antibody

HFE Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17954-1-AP

Synonyms

hemochromatosis, HFE, HFE1, HH, HLA H, HLAH, MVCD7

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Validation Data Gallery

• 

  WB analysis of human placenta using 17954-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17954-1-AP (HFE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 17954-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17954-1-AP (HFE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human placenta tissue, A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17954-1-AP targets HFE in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HFE fusion protein Ag12448
• Full Name: hemochromatosis
• Calculated Molecular Weight: 345 aa, 40 kDa
• Observed Molecular Weight: 45-58 kDa
• GenBank Accession Number: BC074721
• Gene Symbol: HFE
• Gene ID (NCBI): 3077
• RRID: AB_10596779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.