Ferritin light chain Polyclonal antibody

• Featured Product
• KD/KO Validated

Ferritin light chain Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

159

Reviews (2)

Cat no : 10727-1-AP

Synonyms

Ferritin, FTL, Ferritin L subunit

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Validation Data Gallery

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  WB analysis using 10727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10727-1-AP (Ferritin light chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10727-1-AP (Ferritin light chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10727-1-AP (Ferritin light chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10727-1-AP (Ferritin light chain antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10727-1-AP

  IP result of anti-Ferritin light chain (IP:10727-1-AP, 4ug; Detection:10727-1-AP 1:1000) with mouse liver tissue lysate 3040 ug.

• 

  IHC staining of human liver using 10727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10727-1-AP (Ferritin light chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10727-1-AP (Ferritin light chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10727-1-AP (Ferritin light chain antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 10727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10727-1-AP (Ferritin light chain antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse spleen using 10727-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10727-1-AP (Ferritin light chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 10727-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10727-1-AP (Ferritin light chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 10727-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10727-1-AP (Ferritin light chain antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 10727-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10727-1-AP (Ferritin light chain antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 10727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10727-1-AP (Ferritin light chain antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10727-1-AP (Ferritin light chain antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 10727-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Ferritin light chain antibody (10727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 10727-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Ferritin light chain antibody (10727-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human placenta tissue, mouse ovary tissue, mouse testis tissue, mouse spleen tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue, human liver cancer tissue, mouse spleen tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

This antibody is not recommended for WB detection of brain sample.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 137 publications below
• IHC: See 28 publications below
• IF: See 19 publications below

Product Information

10727-1-AP targets Ferritin light chain in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ferritin light chain fusion protein Ag1149
• Full Name: ferritin, light polypeptide
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC008439
• Gene Symbol: Ferritin light chain
• Gene ID (NCBI): 2512
• RRID: AB_2278673
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.

Publications

Species	Application	Title
human,mouse	WB	Cell Metab; Lactate modulates iron metabolism by binding soluble adenylyl cyclase; Authors - Wei Liu
mouse	WB,IHC	Mil Med Res; Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease; Authors - Guang-Hui Deng
human	WB	Nat Cell Biol; Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo.; Authors - William E Dowdle ; KD Validated
mouse	WB,IHC	ACS Nano; Ferroptosis Is Involved in Sex-Specific Small Intestinal Toxicity in the Offspring of Adult Mice Exposed to Polystyrene Nanoplastics during Pregnancy; Authors - Juan Tang
mouse	IF	Nat Commun; Targeting ferroptosis by poly(acrylic) acid coated Mn3O4 nanoparticles alleviates acute liver injury; Authors - Xinyi Shan
human	IHC	Basic Res Cardiol; Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.; Authors - Qiong Lai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Laura (Verified Customer) (08-26-2024)

used for WB

• Applications: Western Blot
• Cell Tissue Type: human

FH

Amandine (Verified Customer) (02-07-2024)

25ug of protein DENATURED, PVDF membrane, primary antibody 1/10000 1h30 RT, secondary antibody 1/5000 2h RT. Chemiluminescence revelation, 6min.

• Applications: Western Blot
• Primary Antibody Dilution: 1/10000
• Cell Tissue Type: ImCC

Ferritin light chain Antibody Western Blot validation (1/10000 dilution) in ImCC (Cat no:10727-1-AP)

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