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  • KD/KO Validated

Ferritin light chain Polyclonal antibody

Ferritin light chain Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10727-1-AP

Synonyms

Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL



Tested Applications

Positive WB detected inmouse liver tissue, human placenta tissue, mouse ovary tissue, mouse testis tissue, mouse spleen tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue, mouse spleen tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10727-1-AP targets Ferritin light chain in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ferritin light chain fusion protein Ag1149
Full Name ferritin, light polypeptide
Calculated molecular weight 19 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC008439
Gene symbol FTL
Gene ID (NCBI) 2512
RRIDAB_2278673
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.

Protocols

Product Specific Protocols
WB protocol for Ferritin light chain antibody 10727-1-APDownload protocol
IHC protocol for Ferritin light chain antibody 10727-1-APDownload protocol
IF protocol for Ferritin light chain antibody 10727-1-APDownload protocol
IP protocol for Ferritin light chain antibody 10727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cell Metab

Lactate modulates iron metabolism by binding soluble adenylyl cyclase

Authors - Wei Liu
mouseWB,IHC

Mil Med Res

Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease

Authors - Guang-Hui Deng
humanWB

Nat Cell Biol

Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo.

Authors - William E Dowdle
  • KD Validated
mouseWB,IHC

ACS Nano

Ferroptosis Is Involved in Sex-Specific Small Intestinal Toxicity in the Offspring of Adult Mice Exposed to Polystyrene Nanoplastics during Pregnancy

Authors - Juan Tang
mouseIF

Nat Commun

Targeting ferroptosis by poly(acrylic) acid coated Mn3O4 nanoparticles alleviates acute liver injury

Authors - Xinyi Shan
humanIHC

Basic Res Cardiol

Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.

Authors - Qiong Lai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Amandine (Verified Customer) (02-07-2024)

25ug of protein DENATURED, PVDF membrane, primary antibody 1/10000 1h30 RT, secondary antibody 1/5000 2h RT. Chemiluminescence revelation, 6min.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/10000
  • Cell Tissue Type: ImCC
Ferritin light chain Antibody Western Blot validation (1/10000 dilution) in ImCC (Cat no:10727-1-AP)