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Ferritin light chain Polyclonal antibody
Ferritin light chain Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 10727-1-AP
Validation Data Gallery
|Positive WB detected in
|mouse liver tissue, human placenta tissue, mouse ovary tissue, mouse testis tissue, mouse spleen tissue, rat liver tissue
|Positive IP detected in
|mouse liver tissue
|Positive IHC detected in
|human liver tissue, human liver cancer tissue, mouse spleen tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10727-1-AP targets Ferritin light chain in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, monkey, duck
|Host / Isotype
|Rabbit / IgG
|Ferritin light chain fusion protein Ag1149
|ferritin, light polypeptide
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.
|Product Specific Protocols
|WB protocol for Ferritin light chain antibody 10727-1-AP
|IHC protocol for Ferritin light chain antibody 10727-1-AP
|IF protocol for Ferritin light chain antibody 10727-1-AP
|IP protocol for Ferritin light chain antibody 10727-1-AP
|Click here to view our Standard Protocols
Lactate modulates iron metabolism by binding soluble adenylyl cyclase
Mil Med Res
Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease
Nat Cell Biol
Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo.
Ferroptosis Is Involved in Sex-Specific Small Intestinal Toxicity in the Offspring of Adult Mice Exposed to Polystyrene Nanoplastics during Pregnancy
Targeting ferroptosis by poly(acrylic) acid coated Mn3O4 nanoparticles alleviates acute liver injury
Basic Res Cardiol
Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Amandine (Verified Customer) (02-07-2024)
25ug of protein DENATURED, PVDF membrane, primary antibody 1/10000 1h30 RT, secondary antibody 1/5000 2h RT. Chemiluminescence revelation, 6min.