Anticorps Polyclonal de lapin anti-TFEB

• Phare
• Validé par KD/KO

TFEB Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay

Conjugaison

Non conjugué

Publications

177

Avis (4)

N° de cat : 13372-1-AP

Synonymes

AlphaTFEB, bHLHe35, TCFEB, TFEB, transcription factor EB

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Galerie de données de validation

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  WB analysis using 13372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13372-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13372-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 13372-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13372-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13372-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 13372-1-AP

  IP result of anti-TFEB (IP:13372-1-AP, 4ug; Detection:13372-1-AP 1:10000) with Raji cells lysate 2800 ug.

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  IHC staining of human renal cell carcinoma using 13372-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 13372-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13372-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TFEB antibody (13372-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules C2C12, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules Raji, cellules RAW 264.7, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu placentaire humain
• Résultats positifs en IP: cellules Raji,
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 27 publications below
• WB: See 148 publications below
• IHC: See 15 publications below
• IF: See 80 publications below
• IP: See 6 publications below
• CoIP: See 2 publications below

Informations sur le produit

13372-1-AP cible TFEB dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, poisson-zèbre, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFEB Protéine recombinante Ag4194
• Nom complet: transcription factor EB
• Masse moléculaire calculée: 476 aa, 53 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC032448
• Symbole du gène: TFEB
• Identification du gène (NCBI): 7942
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

Publications

Species	Application	Title
mouse	WB	ACS Nano; A Self-Assembled α-Synuclein Nanoscavenger for Parkinson's Disease.; Authors - Jingyi Liu
mouse	IF	Acta Neuropathol; Wolframin is a novel regulator of tau pathology and neurodegeneration.; Authors - Shuo Chen
mouse	WB,IF,IP	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB,IF	J Hazard Mater; VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes; Authors - Tao Wang
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang ; KD Validated
mouse	WB	Mol Psychiatry; TFEB regulates lysosomal exocytosis of tau and its loss of function exacerbates tau pathology and spreading.; Authors - Yin Xu ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shunbin (Verified Customer) (07-17-2023)

bands look specific, but blurry and multiple.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: B cell lines

TFEB Antibody Western Blot validation (1:2000 dilution) in B cell lines (Cat no:13372-1-AP)

FH

Weihao (Verified Customer) (04-11-2022)

Good antibody for mouse BMDM! not for human MDM 1:500 for IF 1:2000 for WB

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: mouse BMDM

FH

Marina (Verified Customer) (11-22-2021)

2 bands visible, unclear which is the right one, although size is ok-ish for both. Both bands are quite weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 in 1% skim milk in TBST
• Cell Tissue Type: primary mouse microglia

FH

Tom (Verified Customer) (02-19-2021)

HEK293 lysate (10ug) subjected to SDS-PAGE and transferred to nitrocellullose. Membrane blocked in 5% BSA for 1 hour prior to incubation with antibody (1:1000 in block) overnight at 4 degrees. Band detected using anti-rabbit HRP secondary.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

TFEB Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:13372-1-AP)

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