MITF Polyclonal antibody

MITF Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13092-1-AP

Synonyms

bHLHe32, MI, MITF, WS2A



Tested Applications

Positive WB detected inA549 cells, Jurkat cells, mouse heart tissue, rat skin tissue, NIH/3T3 cells
Positive IP detected inmouse heart tissue
Positive FC detected inhESC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC)FC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13092-1-AP targets MITF in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MITF fusion protein Ag3679
Full Name microphthalmia-associated transcription factor
Calculated molecular weight 91 aa, 10 kDa, 59 kDa
Observed molecular weight 59-65 kDa
GenBank accession numberBC012503
Gene symbol MITF
Gene ID (NCBI) 4286
RRIDAB_10597698
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases[PMID: 22523078]. Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells [PMID: 10578055].

Protocols

Product Specific Protocols
WB protocol for MITF antibody 13092-1-APDownload protocol
IP protocol for MITF antibody 13092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
mouseWB

J Clin Invest

mTORC1 feedback to AKT modulates lysosomal biogenesis through MiT/TFE regulation.

Authors - Kaushal Asrani
mouseWB

Theranostics

TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.

Authors - Kailiang Zhou
human,mouseWB

Phytomedicine

Taxifolin inhibits melanoma proliferation/migration impeding USP18/Rac1/JNK/β-catenin oncogenic signaling

Authors - Libo Xu
human, mouseWB

J Invest Dermatol

PMID: 23223141

Authors - Lee Chang Seok CS
humanWB

Pigment Cell Melanoma Res

D-tyrosine negatively regulates melanin synthesis by competitively inhibiting tyrosinase activity.

Authors - Jisu Park

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alessandro (Verified Customer) (11-06-2022)

no unspecific staining, great outcome

  • Applications: Immunofluorescence